| pubmed-article:20696943 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20696943 | lifeskim:mentions | umls-concept:C0005821 | lld:lifeskim |
| pubmed-article:20696943 | lifeskim:mentions | umls-concept:C0032173 | lld:lifeskim |
| pubmed-article:20696943 | lifeskim:mentions | umls-concept:C0042971 | lld:lifeskim |
| pubmed-article:20696943 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:20696943 | lifeskim:mentions | umls-concept:C2936194 | lld:lifeskim |
| pubmed-article:20696943 | pubmed:issue | 19 | lld:pubmed |
| pubmed-article:20696943 | pubmed:dateCreated | 2010-11-12 | lld:pubmed |
| pubmed-article:20696943 | pubmed:abstractText | The function of the mechanosensitive, multimeric blood protein von Willebrand factor (VWF) is dependent on its size. We tested the hypothesis that VWF may self-associate on the platelet glycoprotein Ib? (GpIb?) receptor under hydrodynamic shear. Consistent with this proposition, whereas Alexa-488-conjugated VWF (VWF-488) bound platelets at modest levels, addition of unlabeled VWF enhanced the extent of VWF-488 binding. Recombinant VWF lacking the A1-domain was conjugated with Alexa-488 to produce ?A1-488. Although ?A1-488 alone did not bind platelets under shear, this protein bound GpIb? on addition of either purified plasma VWF or recombinant full-length VWF. The extent of self-association increased with applied shear stress more than ? 60 to 70 dyne/cm(2). ?A1-488 bound platelets in the milieu of plasma. On application of fluid shear to whole blood, half of the activated platelets had ?A1-488 bound, suggesting that VWF self-association may be necessary for cell activation. Shearing platelets with 6-?m beads bearing either immobilized VWF or anti-GpIb? mAb resulted in cell activation at shear stress down to 2 to 5 dyne/cm(2). Taken together, the data suggest that fluid shear in circulation can increase the effective size of VWF bound to platelet GpIb? via protein self-association. This can trigger mechanotransduction and cell activation by enhancing the drag force applied on the cell-surface receptor. | lld:pubmed |
| pubmed-article:20696943 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:20696943 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20696943 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20696943 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20696943 | pubmed:citationSubset | AIM | lld:pubmed |
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| pubmed-article:20696943 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20696943 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20696943 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:20696943 | pubmed:issn | 1528-0020 | lld:pubmed |
| pubmed-article:20696943 | pubmed:author | pubmed-author:NeelameghamSr... | lld:pubmed |
| pubmed-article:20696943 | pubmed:author | pubmed-author:DayanandaKann... | lld:pubmed |
| pubmed-article:20696943 | pubmed:author | pubmed-author:SinghIndrajee... | lld:pubmed |
| pubmed-article:20696943 | pubmed:author | pubmed-author:MondalNandini... | lld:pubmed |
| pubmed-article:20696943 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20696943 | pubmed:day | 11 | lld:pubmed |
| pubmed-article:20696943 | pubmed:volume | 116 | lld:pubmed |
| pubmed-article:20696943 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20696943 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20696943 | pubmed:pagination | 3990-8 | lld:pubmed |
| pubmed-article:20696943 | pubmed:dateRevised | 2011-11-14 | lld:pubmed |
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| pubmed-article:20696943 | pubmed:meshHeading | pubmed-meshheading:20696943... | lld:pubmed |
| pubmed-article:20696943 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20696943 | pubmed:articleTitle | von Willebrand factor self-association on platelet GpIbalpha under hydrodynamic shear: effect on shear-induced platelet activation. | lld:pubmed |
| pubmed-article:20696943 | pubmed:affiliation | Chemical and Biological Engineering, State University of New York, Buffalo, NY 14260, USA. | lld:pubmed |
| pubmed-article:20696943 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20696943 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:20696943 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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