| pubmed-article:20683158 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20683158 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:20683158 | lifeskim:mentions | umls-concept:C0220908 | lld:lifeskim |
| pubmed-article:20683158 | lifeskim:mentions | umls-concept:C0806140 | lld:lifeskim |
| pubmed-article:20683158 | lifeskim:mentions | umls-concept:C0450442 | lld:lifeskim |
| pubmed-article:20683158 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:20683158 | lifeskim:mentions | umls-concept:C0179038 | lld:lifeskim |
| pubmed-article:20683158 | lifeskim:mentions | umls-concept:C1510941 | lld:lifeskim |
| pubmed-article:20683158 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:20683158 | pubmed:dateCreated | 2010-8-4 | lld:pubmed |
| pubmed-article:20683158 | pubmed:abstractText | New in vitro methods are desirable for the analysis of platelet aggregation and screening novel anti-platelet agents using whole blood. To this end, we examined platelet aggregation and thrombus formation in whole human blood from healthy volunteers using a microchannel array flow analyzer (MC-FAN). Platelet aggregation in whole blood, treated with the activating agents ADP, collagen or ristocetin was detected in the MC-FAN by measuring the decrease in flow rate as a function of agent concentration. The results were compared with aggregation in platelet rich plasma (PRP) in a conventional aggregometer, as measured by the increase in optical density. The MC-FAN detected platelet aggregation in whole blood at two- to four-fold lower concentrations of agonist compared to those in PRP in the aggregometer. Anti-platelet agents attenuated the decrease in blood flow rate in the MC-FAN by inhibiting fibrin formation and platelet aggregation, but anticoagulants only inhibited fibrin formation and did not affect blood flow rates. These findings suggest that the MC-FAN system may be a useful method for the evaluation of platelet activation and facilitate the development of novel anti-platelet agents. | lld:pubmed |
| pubmed-article:20683158 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20683158 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20683158 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20683158 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20683158 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20683158 | pubmed:issn | 1878-5034 | lld:pubmed |
| pubmed-article:20683158 | pubmed:author | pubmed-author:SuzukiKojiK | lld:pubmed |
| pubmed-article:20683158 | pubmed:author | pubmed-author:HayashiTatsuy... | lld:pubmed |
| pubmed-article:20683158 | pubmed:author | pubmed-author:OkamotoTakayu... | lld:pubmed |
| pubmed-article:20683158 | pubmed:author | pubmed-author:KamadaHaruhik... | lld:pubmed |
| pubmed-article:20683158 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20683158 | pubmed:volume | 47 | lld:pubmed |
| pubmed-article:20683158 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20683158 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20683158 | pubmed:pagination | 153-61 | lld:pubmed |
| pubmed-article:20683158 | pubmed:dateRevised | 2010-12-2 | lld:pubmed |
| pubmed-article:20683158 | pubmed:meshHeading | pubmed-meshheading:20683158... | lld:pubmed |
| pubmed-article:20683158 | pubmed:meshHeading | pubmed-meshheading:20683158... | lld:pubmed |
| pubmed-article:20683158 | pubmed:meshHeading | pubmed-meshheading:20683158... | lld:pubmed |
| pubmed-article:20683158 | pubmed:meshHeading | pubmed-meshheading:20683158... | lld:pubmed |
| pubmed-article:20683158 | pubmed:meshHeading | pubmed-meshheading:20683158... | lld:pubmed |
| pubmed-article:20683158 | pubmed:meshHeading | pubmed-meshheading:20683158... | lld:pubmed |
| pubmed-article:20683158 | pubmed:meshHeading | pubmed-meshheading:20683158... | lld:pubmed |
| pubmed-article:20683158 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20683158 | pubmed:articleTitle | An in vitro method for screening anti-platelet agents using a microchannel array flow analyzer. | lld:pubmed |
| pubmed-article:20683158 | pubmed:affiliation | Department of Molecular Pathobiology, Mie University School of Medicine, Mie, Osaka, Japan. kamada@nibio.go.jp | lld:pubmed |
| pubmed-article:20683158 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20683158 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
