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pubmed-article:20600935pubmed:abstractTextCurrent antiretroviral therapies would greatly benefit from the concurrent removal of integrated HIV-1 proviral DNA from the patient's cells. In this review, we describe an experimental strategy that allowed the engineering and functional analysis of a HIV-1 LTR-specific recombinase (Tre-recombinase). We furthermore provide protocols that are utilized for the investigation of Tre's antiretroviral activity in infected tissue cultures as well as in infected humanized Rag2(-/-)?c(-/-) mice.lld:pubmed
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pubmed-article:20600935pubmed:authorpubmed-author:HauberJoachim...lld:pubmed
pubmed-article:20600935pubmed:authorpubmed-author:BuchholzFrank...lld:pubmed
pubmed-article:20600935pubmed:copyrightInfoCopyright © 2010 Elsevier Inc. All rights reserved.lld:pubmed
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pubmed-article:20600935pubmed:articleTitleIn vitro evolution and analysis of HIV-1 LTR-specific recombinases.lld:pubmed
pubmed-article:20600935pubmed:affiliationMax-Planck-Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.lld:pubmed
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