| pubmed-article:20599757 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20599757 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:20599757 | lifeskim:mentions | umls-concept:C0205107 | lld:lifeskim |
| pubmed-article:20599757 | lifeskim:mentions | umls-concept:C0022702 | lld:lifeskim |
| pubmed-article:20599757 | lifeskim:mentions | umls-concept:C1819241 | lld:lifeskim |
| pubmed-article:20599757 | lifeskim:mentions | umls-concept:C2700087 | lld:lifeskim |
| pubmed-article:20599757 | lifeskim:mentions | umls-concept:C0851827 | lld:lifeskim |
| pubmed-article:20599757 | lifeskim:mentions | umls-concept:C1701901 | lld:lifeskim |
| pubmed-article:20599757 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:20599757 | pubmed:dateCreated | 2010-8-3 | lld:pubmed |
| pubmed-article:20599757 | pubmed:abstractText | The presence of high and low sodium affinity states for the Na(+)-dependent [(14)C]-l-alanine uptake in immortalized renal proximal tubular epithelial (PTE) cells was previously reported (Am. J. Physiol. 293 (2007) R538-R547). This study evaluated the role of H(2)O(2) on the Na(+)-dependent [(14)C]-l-alanine uptake of ASCT2 in immortalized renal PTE cells from Wistar Kyoto rat (WKY) and spontaneously hypertensive rat (SHR). Na(+) dependence of [(14)C]-l-alanine uptake was investigated replacing NaCl with an equimolar concentration of choline chloride in vehicle- and apocynin-treated cells. Na(+) removal from the uptake solution abolished transport activity in both WKY and SHR PTE cells. Decreases in H(2)O(2) levels in the extracellular medium significantly reduced Na(+)-K(m) and V(max) values of the low-affinity high-capacity component in SHR PTE cells, with no effect on the high-affinity low-capacity state of the Na(+)-dependent [(14)C]-l-alanine uptake. After removal of apocynin from the culture medium, H(2)O(2) levels returned to basal values within 1 to 3h in both WKY and SHR PTE cells and these were found stable for the next 24h. Under these experimental conditions, the Na(+)-K(m) and V(max) of the high-affinity low-capacity state were unaffected and the low-affinity high-capacity component remained significantly decreased 1day but not 4days after apocynin removal. In conclusion, H(2)O(2) in excess is required for the presence of a low-affinity high-capacity component for the Na(+)-dependent [(14)C]-l-alanine uptake in SHR PTE cells only. It is suggested that Na(+) binding in renal ASCT2 may be regulated by ROS in SHR PTE cells. | lld:pubmed |
| pubmed-article:20599757 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20599757 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20599757 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20599757 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20599757 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20599757 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20599757 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20599757 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20599757 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20599757 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:20599757 | pubmed:issn | 1090-2104 | lld:pubmed |
| pubmed-article:20599757 | pubmed:author | pubmed-author:JosePedro APA | lld:pubmed |
| pubmed-article:20599757 | pubmed:author | pubmed-author:Soares-da-Sil... | lld:pubmed |
| pubmed-article:20599757 | pubmed:author | pubmed-author:PinhoMaria... | lld:pubmed |
| pubmed-article:20599757 | pubmed:author | pubmed-author:PintoVandaV | lld:pubmed |
| pubmed-article:20599757 | pubmed:copyrightInfo | Copyright 2010 Elsevier Inc. All rights reserved. | lld:pubmed |
| pubmed-article:20599757 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20599757 | pubmed:day | 30 | lld:pubmed |
| pubmed-article:20599757 | pubmed:volume | 398 | lld:pubmed |
| pubmed-article:20599757 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20599757 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20599757 | pubmed:pagination | 553-8 | lld:pubmed |
| pubmed-article:20599757 | pubmed:meshHeading | pubmed-meshheading:20599757... | lld:pubmed |
| pubmed-article:20599757 | pubmed:meshHeading | pubmed-meshheading:20599757... | lld:pubmed |
| pubmed-article:20599757 | pubmed:meshHeading | pubmed-meshheading:20599757... | lld:pubmed |
| pubmed-article:20599757 | pubmed:meshHeading | pubmed-meshheading:20599757... | lld:pubmed |
| pubmed-article:20599757 | pubmed:meshHeading | pubmed-meshheading:20599757... | lld:pubmed |
| pubmed-article:20599757 | pubmed:meshHeading | pubmed-meshheading:20599757... | lld:pubmed |
| pubmed-article:20599757 | pubmed:meshHeading | pubmed-meshheading:20599757... | lld:pubmed |
| pubmed-article:20599757 | pubmed:meshHeading | pubmed-meshheading:20599757... | lld:pubmed |
| pubmed-article:20599757 | pubmed:meshHeading | pubmed-meshheading:20599757... | lld:pubmed |
| pubmed-article:20599757 | pubmed:meshHeading | pubmed-meshheading:20599757... | lld:pubmed |
| pubmed-article:20599757 | pubmed:meshHeading | pubmed-meshheading:20599757... | lld:pubmed |
| pubmed-article:20599757 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20599757 | pubmed:articleTitle | Role of H(2)O(2) on the kinetics of low-affinity high-capacity Na(+)-dependent alanine transport in SHR proximal tubular epithelial cells. | lld:pubmed |
| pubmed-article:20599757 | pubmed:affiliation | Institute of Pharmacology and Therapeutics, Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal. | lld:pubmed |
| pubmed-article:20599757 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20599757 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
