| pubmed-article:2056007 | pubmed:abstractText | A high-performance liquid chromatographic assay with solid-phase extraction (SPE) for the rapid and sensitive quantitation of 3-(5-cyclopropyl-1,2,4-oxadiazol-3-yl)-5-(1-methylethyl)imidazo[1, 5-a]quinoxalin-4(5H)-one (I, U-78875) in serum is described. The validation results indicated that the present method had excellent intra- and inter-assay precision (less than or equal to 9.5%, mean +/- S.D. = 3.9 +/- 3.0%, n = 25) and accuracy (less than or equal to 10.0%, mean +/- S.D. = 3.0 +/- 2.9%, n = 25), as well as improved sensitivity (2 ng ml, using a 100-microliters injection). Each chromatographic run is only 10 min and the organic solvent for the extraction of I and internal standard (U-82217) from serum was only 300 microliters. The application results obtained from the SPE method were in good agreement with the advanced automated sample preparation method. | lld:pubmed |
