| pubmed-article:20535218 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20535218 | lifeskim:mentions | umls-concept:C1704632 | lld:lifeskim |
| pubmed-article:20535218 | lifeskim:mentions | umls-concept:C0871261 | lld:lifeskim |
| pubmed-article:20535218 | lifeskim:mentions | umls-concept:C0334094 | lld:lifeskim |
| pubmed-article:20535218 | lifeskim:mentions | umls-concept:C2911692 | lld:lifeskim |
| pubmed-article:20535218 | lifeskim:mentions | umls-concept:C1706817 | lld:lifeskim |
| pubmed-article:20535218 | lifeskim:mentions | umls-concept:C0439662 | lld:lifeskim |
| pubmed-article:20535218 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:20535218 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:20535218 | lifeskim:mentions | umls-concept:C0205195 | lld:lifeskim |
| pubmed-article:20535218 | lifeskim:mentions | umls-concept:C0443252 | lld:lifeskim |
| pubmed-article:20535218 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:20535218 | pubmed:dateCreated | 2010-11-11 | lld:pubmed |
| pubmed-article:20535218 | pubmed:abstractText | Several vaccination trials are evaluating the modified vaccinia virus Ankara (MVA) as a delivery vector in various clinical settings. In this paper, we present the reevaluation of a therapeutic vaccination trial in human immunodeficiency virus (HIV)-1-infected individuals treated with highly active antiretroviral therapy using MVA-expressing HIV-1 nef. Immunogenicity of MVA-nef was assessed using multicolor flow cytometry. Vaccine-induced polyfunctionality and proliferative capacity, which are associated with nonprogressive HIV-1 infection, were detectable by combining two immune assays. By means of short-term polychromatic intracellular cytokine staining, we observed a significant increase in polyfunctional Nef-specific CD4 T cells expressing interferon-?, interleukin (IL)-2 and CD154 after vaccination, whereas changes in the quality of CD8 T-cell response could not be observed. Only the additional use of a long-term polychromatic Carboxyfluorescein succinimidyl ester (CFSE)-based proliferation assay revealed vaccine-induced Nef-specific CD8, as well as CD4 T cells with proliferative capacity. The correlation between vaccine-induced IL-2 production by CD4 T cells and the increase in proliferating Nef-specific CD8 T cells suggests a causal link between these two functions. These results highlight the importance of combining sophisticated immunomonitoring tools to unravel concealed effects of immunological interventions and support the use of the poxvirus-derived MVA vector to stimulate highly functional HIV-1-specific T-cell responses. However, the clinical benefit of these functional T cells remains to be determined. | lld:pubmed |
| pubmed-article:20535218 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20535218 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20535218 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20535218 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20535218 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20535218 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20535218 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20535218 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20535218 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20535218 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20535218 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:20535218 | pubmed:issn | 1476-5462 | lld:pubmed |
| pubmed-article:20535218 | pubmed:author | pubmed-author:GoebelF DFD | lld:pubmed |
| pubmed-article:20535218 | pubmed:author | pubmed-author:ErfleVV | lld:pubmed |
| pubmed-article:20535218 | pubmed:author | pubmed-author:CosmaAA | lld:pubmed |
| pubmed-article:20535218 | pubmed:author | pubmed-author:BognerJ RJR | lld:pubmed |
| pubmed-article:20535218 | pubmed:author | pubmed-author:ProtzerUU | lld:pubmed |
| pubmed-article:20535218 | pubmed:author | pubmed-author:KutscherSS | lld:pubmed |
| pubmed-article:20535218 | pubmed:author | pubmed-author:AllgayerSS | lld:pubmed |
| pubmed-article:20535218 | pubmed:author | pubmed-author:DembekC JCJ | lld:pubmed |
| pubmed-article:20535218 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20535218 | pubmed:volume | 17 | lld:pubmed |
| pubmed-article:20535218 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20535218 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20535218 | pubmed:pagination | 1372-83 | lld:pubmed |
| pubmed-article:20535218 | pubmed:meshHeading | pubmed-meshheading:20535218... | lld:pubmed |
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| pubmed-article:20535218 | pubmed:meshHeading | pubmed-meshheading:20535218... | lld:pubmed |
| pubmed-article:20535218 | pubmed:meshHeading | pubmed-meshheading:20535218... | lld:pubmed |
| pubmed-article:20535218 | pubmed:meshHeading | pubmed-meshheading:20535218... | lld:pubmed |
| pubmed-article:20535218 | pubmed:meshHeading | pubmed-meshheading:20535218... | lld:pubmed |
| pubmed-article:20535218 | pubmed:meshHeading | pubmed-meshheading:20535218... | lld:pubmed |
| pubmed-article:20535218 | pubmed:meshHeading | pubmed-meshheading:20535218... | lld:pubmed |
| pubmed-article:20535218 | pubmed:meshHeading | pubmed-meshheading:20535218... | lld:pubmed |
| pubmed-article:20535218 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20535218 | pubmed:articleTitle | MVA-nef induces HIV-1-specific polyfunctional and proliferative T-cell responses revealed by the combination of short- and long-term immune assays. | lld:pubmed |
| pubmed-article:20535218 | pubmed:affiliation | Institute of Virology, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany. sarah.kutscher@helmholtz-muenchen.de | lld:pubmed |
| pubmed-article:20535218 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20535218 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:20535218 | lld:entrezgene |
