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pubmed-article:20519455pubmed:abstractTextComplete genome sequences were determined for two distinct strains of slow bee paralysis virus (SBPV) of honeybees (Apis mellifera). The SBPV genome is approximately 9.5 kb long and contains a single ORF flanked by 5'- and 3'-UTRs and a naturally polyadenylated 3' tail, with a genome organization typical of members of the family Iflaviridae. The two strains, labelled 'Rothamsted' and 'Harpenden', are 83% identical at the nucleotide level (94% identical at the amino acid level), although this variation is distributed unevenly over the genome. The two strains were found to co-exist at different proportions in two independently propagated SBPV preparations. The natural prevalence of SBPV for 847 colonies in 162 apiaries across five European countries was <2%, with positive samples found only in England and Switzerland, in colonies with variable degrees of Varroa infestation.lld:pubmed
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pubmed-article:20519455pubmed:articleTitleGenetic characterization of slow bee paralysis virus of the honeybee (Apis mellifera L.).lld:pubmed
pubmed-article:20519455pubmed:affiliationSchool of Biological Sciences, Queen's University Belfast, Belfast BT9 7BL, Republic of Ireland. joachim.de.miranda@ekol.slu.selld:pubmed
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