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pubmed-article:20495031pubmed:abstractTextA fructophilic lactic acid bacterium, designated strain F214-1(T), was isolated from a flower of Tropaeolum majus in South Africa. Based on phylogenetic analysis of 16S rRNA gene sequences, the strain formed a subcluster with Fructobacillus ficulneus and Fructobacillus pseudoficulneus and, based on recA gene sequences, the strain formed a subcluster with F. ficulneus. DNA-DNA hybridization studies showed that strain F214-1(T) was phylogenetically distinct from its closest relatives. Acid was produced from the fermentation of d-glucose, d-fructose and d-mannitol only. d-Fructose was the preferred sole carbon and energy source and was fermented more rapidly than d-glucose. Growth of the strain on d-glucose under anaerobic conditions was very weak but external electron acceptors such as oxygen and pyruvate enhanced growth on d-glucose. Lactic acid and acetic acid were produced from d-glucose in equimolar amounts. Ethanol was produced at very low levels, despite the strain's obligately heterofermentative metabolism. Based on these data, strain F214-1(T) represents a novel species of fructophilic bacteria in the genus Fructobacillus, for which the name Fructobacillus tropaeoli sp. nov. is proposed. The type strain is F214-1(T) (?=?JCM 16675(T) ?=?DSM 23246(T)).lld:pubmed
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pubmed-article:20495031pubmed:articleTitleFructobacillus tropaeoli sp. nov., a fructophilic lactic acid bacterium isolated from a flower.lld:pubmed
pubmed-article:20495031pubmed:affiliationDepartment of Microbiology, University of Stellenbosch, Stellenbosch 7600, South Africa. pegaman@hotmail.co.jplld:pubmed
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