Source:http://linkedlifedata.com/resource/pubmed/id/20408650
| Subject | Predicate | Object | Context |
|---|---|---|---|
| pubmed-article:20408650 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20408650 | lifeskim:mentions | umls-concept:C0020944 | lld:lifeskim |
| pubmed-article:20408650 | lifeskim:mentions | umls-concept:C0032521 | lld:lifeskim |
| pubmed-article:20408650 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:20408650 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:20408650 | pubmed:dateCreated | 2010-4-22 | lld:pubmed |
| pubmed-article:20408650 | pubmed:abstractText | The authors report a facile method for the selective immobilization of biomolecules onto a gold surface that was preactivated by a polymeric adlayer. The polymeric adlayer was designed to perform triple functions: high resistance to nonspecific protein adsorption, efficient surface anchoring, and subsequent covalent attachment of biomolecules. For this purpose, a random copolymer, poly(PEGMA-r-NAS), was synthesized by radical polymerization of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and N-acryloxysuccinimide (NAS). In the first step, the polymeric adlayer was formed onto amine-terminated self-assembled monolayers (SAMs) on gold through covalent bond formation between reactive N-hydroxysuccinimide (NHS) ester of the copolymer and the amine of the SAMs. In the second step, amine-bearing biotin as a model biomolecule was covalently attached onto the polymeric adlayer that still contained unreacted NHS esters. The degrees of the binding sensitivity for a target protein and the nonspecific binding for four model proteins on the biotinylated polymeric adlayer were examined by surface plasmon resonance spectroscopy. Finally, the specific immobilization of rhodamin (TRITC)-conjugated streptavidin on the biotinylated polymeric adlayer was achieved by a simple microcontact printing technique, resulting in well-defined patterns of the protein. | lld:pubmed |
| pubmed-article:20408650 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20408650 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20408650 | pubmed:status | PubMed-not-MEDLINE | lld:pubmed |
| pubmed-article:20408650 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:20408650 | pubmed:issn | 1559-4106 | lld:pubmed |
| pubmed-article:20408650 | pubmed:author | pubmed-author:ChoiInsung... | lld:pubmed |
| pubmed-article:20408650 | pubmed:author | pubmed-author:LeeKyung-BokK... | lld:pubmed |
| pubmed-article:20408650 | pubmed:author | pubmed-author:ParkSangjinS | lld:pubmed |
| pubmed-article:20408650 | pubmed:author | pubmed-author:LeeBong SooBS | lld:pubmed |
| pubmed-article:20408650 | pubmed:author | pubmed-author:JonSangyongS | lld:pubmed |
| pubmed-article:20408650 | pubmed:copyrightInfo | 2007 American Vacuum Society. | lld:pubmed |
| pubmed-article:20408650 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20408650 | pubmed:volume | 2 | lld:pubmed |
| pubmed-article:20408650 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20408650 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20408650 | pubmed:pagination | 136-42 | lld:pubmed |
| pubmed-article:20408650 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:20408650 | pubmed:articleTitle | Selective immobilization of biomolecules onto an activated polymeric adlayer. | lld:pubmed |
| pubmed-article:20408650 | pubmed:affiliation | Department of Chemistry and School of Molecular Science (BK21), Center for Molecular Design and Synthesis, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Korea. | lld:pubmed |
| pubmed-article:20408650 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20408650 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |