| pubmed-article:2037295 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2037295 | lifeskim:mentions | umls-concept:C0023621 | lld:lifeskim |
| pubmed-article:2037295 | lifeskim:mentions | umls-concept:C0009013 | lld:lifeskim |
| pubmed-article:2037295 | lifeskim:mentions | umls-concept:C0008633 | lld:lifeskim |
| pubmed-article:2037295 | lifeskim:mentions | umls-concept:C0184511 | lld:lifeskim |
| pubmed-article:2037295 | lifeskim:mentions | umls-concept:C0204727 | lld:lifeskim |
| pubmed-article:2037295 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:2037295 | lifeskim:mentions | umls-concept:C0036576 | lld:lifeskim |
| pubmed-article:2037295 | lifeskim:mentions | umls-concept:C0013862 | lld:lifeskim |
| pubmed-article:2037295 | lifeskim:mentions | umls-concept:C0205485 | lld:lifeskim |
| pubmed-article:2037295 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:2037295 | pubmed:dateCreated | 1991-7-2 | lld:pubmed |
| pubmed-article:2037295 | pubmed:abstractText | We had previously developed an efficient procedure for selective cloning of rare-cutter linking fragments that is based on physical separation of linking clone DNAs by pulsed-field polyacrylamide gel electrophoresis (PF-PAGE). An advantage of the physical selection procedure over the conventional cloning-based ones utilizing the insertion of selection marker or vector sequences into the rare-cutter sites is that it can be readily applied to the selection of linking fragments for rare-cutters, generating ambiguous cohesive end sequences such as SfiI (GGCCNNNN/NGGCC). In the present work, the physical separation procedure was improved by introducing a discontinuous buffer system into PF-PAGE, and its feasibility was exemplified by the selective isolation of SfiI linking clones from a human chromosome 21-specific library. This simple and efficient procedure will provide a useful tool for genome analysis. | lld:pubmed |
| pubmed-article:2037295 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2037295 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2037295 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2037295 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2037295 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2037295 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2037295 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2037295 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2037295 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:2037295 | pubmed:issn | 0888-7543 | lld:pubmed |
| pubmed-article:2037295 | pubmed:author | pubmed-author:ItoTT | lld:pubmed |
| pubmed-article:2037295 | pubmed:author | pubmed-author:SakakiYY | lld:pubmed |
| pubmed-article:2037295 | pubmed:author | pubmed-author:OishiNN | lld:pubmed |
| pubmed-article:2037295 | pubmed:author | pubmed-author:OhgusuHH | lld:pubmed |
| pubmed-article:2037295 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2037295 | pubmed:volume | 9 | lld:pubmed |
| pubmed-article:2037295 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2037295 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2037295 | pubmed:pagination | 707-12 | lld:pubmed |
| pubmed-article:2037295 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
| pubmed-article:2037295 | pubmed:meshHeading | pubmed-meshheading:2037295-... | lld:pubmed |
| pubmed-article:2037295 | pubmed:meshHeading | pubmed-meshheading:2037295-... | lld:pubmed |
| pubmed-article:2037295 | pubmed:meshHeading | pubmed-meshheading:2037295-... | lld:pubmed |
| pubmed-article:2037295 | pubmed:meshHeading | pubmed-meshheading:2037295-... | lld:pubmed |
| pubmed-article:2037295 | pubmed:meshHeading | pubmed-meshheading:2037295-... | lld:pubmed |
| pubmed-article:2037295 | pubmed:meshHeading | pubmed-meshheading:2037295-... | lld:pubmed |
| pubmed-article:2037295 | pubmed:meshHeading | pubmed-meshheading:2037295-... | lld:pubmed |
| pubmed-article:2037295 | pubmed:meshHeading | pubmed-meshheading:2037295-... | lld:pubmed |
| pubmed-article:2037295 | pubmed:meshHeading | pubmed-meshheading:2037295-... | lld:pubmed |
| pubmed-article:2037295 | pubmed:meshHeading | pubmed-meshheading:2037295-... | lld:pubmed |
| pubmed-article:2037295 | pubmed:meshHeading | pubmed-meshheading:2037295-... | lld:pubmed |
| pubmed-article:2037295 | pubmed:meshHeading | pubmed-meshheading:2037295-... | lld:pubmed |
| pubmed-article:2037295 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:2037295 | pubmed:articleTitle | An improved pulsed-field polyacrylamide gel electrophoresis system for physical selection of linking clones: isolation of SfiI linking clones from a chromosome 21-specific library. | lld:pubmed |
| pubmed-article:2037295 | pubmed:affiliation | Department of Preventive Medicine, Nagasaki University, Japan. | lld:pubmed |
| pubmed-article:2037295 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2037295 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2037295 | lld:pubmed |
