| pubmed-article:20337396 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20337396 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:20337396 | lifeskim:mentions | umls-concept:C0031694 | lld:lifeskim |
| pubmed-article:20337396 | lifeskim:mentions | umls-concept:C0201699 | lld:lifeskim |
| pubmed-article:20337396 | lifeskim:mentions | umls-concept:C0332324 | lld:lifeskim |
| pubmed-article:20337396 | pubmed:issue | 15 | lld:pubmed |
| pubmed-article:20337396 | pubmed:dateCreated | 2010-3-26 | lld:pubmed |
| pubmed-article:20337396 | pubmed:abstractText | Enantioselective phosphorescence lifetime detection was combined with chiral cyclodextrin-based electrokinetic chromatography for the analysis of camphorquinone (CQ). A time-gated detection system based on a pulsed light-emitting diode for excitation at 465 nm was developed for the online lifetime determination. The background electrolyte for the chiral separation consisted of 20 mM alpha-cyclodextrin (alpha-CD), 10 mM carboxymethyl-beta-CD, and 25 mM borate buffer at pH 9.0. The separation of (+)-CQ and (-)-CQ is caused by a difference in association constants of these enantiomers with alpha-CD. Under the separation conditions, different phosphorescence lifetimes were obtained for (+)-CQ and (-)-CQ (tau = 384 +/- 8 and 143 +/- 5 micros, respectively), which could be used to distinguish the enantiomers. This selectivity in detection is based on a difference in protection of the enantiomers against phosphorescence quenching after their complexation with alpha-CD. Concentration detection limits were 2 x 10(-7) and 1 x 10(-6) M for (+)-CQ and (-)-CQ, respectively. After correction for the lifetime shortening by triplet-triplet annihilation at higher CQ concentrations, a linear dynamic range was obtained from the detection limit up to 2 mM. The system was used to determine the enantiomeric impurity levels of commercial samples of (+)-CQ and (-)-CQ; 0.2% and 0.1%, respectively. | lld:pubmed |
| pubmed-article:20337396 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20337396 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20337396 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20337396 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20337396 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20337396 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20337396 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20337396 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20337396 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20337396 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20337396 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:20337396 | pubmed:issn | 1520-6882 | lld:pubmed |
| pubmed-article:20337396 | pubmed:author | pubmed-author:van der... | lld:pubmed |
| pubmed-article:20337396 | pubmed:author | pubmed-author:ArieseFreekF | lld:pubmed |
| pubmed-article:20337396 | pubmed:author | pubmed-author:GooijerCeesC | lld:pubmed |
| pubmed-article:20337396 | pubmed:author | pubmed-author:BuijsJoostJ | lld:pubmed |
| pubmed-article:20337396 | pubmed:author | pubmed-author:LammersIvonne... | lld:pubmed |
| pubmed-article:20337396 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20337396 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:20337396 | pubmed:volume | 81 | lld:pubmed |
| pubmed-article:20337396 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20337396 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20337396 | pubmed:pagination | 6226-33 | lld:pubmed |
| pubmed-article:20337396 | pubmed:meshHeading | pubmed-meshheading:20337396... | lld:pubmed |
| pubmed-article:20337396 | pubmed:meshHeading | pubmed-meshheading:20337396... | lld:pubmed |
| pubmed-article:20337396 | pubmed:meshHeading | pubmed-meshheading:20337396... | lld:pubmed |
| pubmed-article:20337396 | pubmed:meshHeading | pubmed-meshheading:20337396... | lld:pubmed |
| pubmed-article:20337396 | pubmed:meshHeading | pubmed-meshheading:20337396... | lld:pubmed |
| pubmed-article:20337396 | pubmed:meshHeading | pubmed-meshheading:20337396... | lld:pubmed |
| pubmed-article:20337396 | pubmed:meshHeading | pubmed-meshheading:20337396... | lld:pubmed |
| pubmed-article:20337396 | pubmed:meshHeading | pubmed-meshheading:20337396... | lld:pubmed |
| pubmed-article:20337396 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:20337396 | pubmed:articleTitle | Phosphorescence for sensitive enantioselective detection in chiral capillary electrophoresis. | lld:pubmed |
| pubmed-article:20337396 | pubmed:affiliation | Department of Analytical Chemistry and Applied Spectroscopy, Laser Centre Vrije Universiteit Amsterdam, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands. | lld:pubmed |
| pubmed-article:20337396 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20337396 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:20337396 | lld:pubmed |
