| pubmed-article:20331971 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20331971 | lifeskim:mentions | umls-concept:C0178539 | lld:lifeskim |
| pubmed-article:20331971 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
| pubmed-article:20331971 | lifeskim:mentions | umls-concept:C1413287 | lld:lifeskim |
| pubmed-article:20331971 | lifeskim:mentions | umls-concept:C2362057 | lld:lifeskim |
| pubmed-article:20331971 | lifeskim:mentions | umls-concept:C0470187 | lld:lifeskim |
| pubmed-article:20331971 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:20331971 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:20331971 | pubmed:dateCreated | 2010-4-19 | lld:pubmed |
| pubmed-article:20331971 | pubmed:abstractText | Viral K-cyclin derived from Kaposi's sarcoma-associated herpesvirus is homologous with mammalian D-type cyclins. Here, we demonstrated the regulatory mechanisms for K-cyclin function and degradation in human embryonic kidney HEK293 and primary effusion lymphoma JSC-1 cell lines. Proteasome inhibitor MG132 treatment induced an accumulation of ubiquitinated K-cyclin in these cells, and co-expression of CDK6 prevented K-cyclin ubiquitination. Also K-cyclin mutants incompetent for CDK6-binding were destabilized by proteasome pathway. Furthermore, silencing of p16INK4a promoted K-cyclin-CDK6 complex formation and hence induced K-cyclin-associated kinase activity in HEK293 cells. These observations indicate that CDK6-bound K-cyclin is functionally stable but monomeric K-cyclin is targeted to ubiquitin-dependent degradation pathway in these cells. Our data suggest that the balance between CDK6 and p16INK4a regulates the availability of functional K-cyclin in human cells. | lld:pubmed |
| pubmed-article:20331971 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20331971 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20331971 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20331971 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20331971 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20331971 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20331971 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20331971 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20331971 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20331971 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20331971 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:20331971 | pubmed:issn | 1090-2104 | lld:pubmed |
| pubmed-article:20331971 | pubmed:author | pubmed-author:SugimotoYoshi... | lld:pubmed |
| pubmed-article:20331971 | pubmed:author | pubmed-author:NoguchiKohjiK | lld:pubmed |
| pubmed-article:20331971 | pubmed:author | pubmed-author:KatayamaKazuh... | lld:pubmed |
| pubmed-article:20331971 | pubmed:author | pubmed-author:YamagoeSatosh... | lld:pubmed |
| pubmed-article:20331971 | pubmed:author | pubmed-author:FujimuroMasah... | lld:pubmed |
| pubmed-article:20331971 | pubmed:author | pubmed-author:MitsuhashiJun... | lld:pubmed |
| pubmed-article:20331971 | pubmed:author | pubmed-author:YoshiokaHiden... | lld:pubmed |
| pubmed-article:20331971 | pubmed:copyrightInfo | 2010 Elsevier Inc. All rights reserved. | lld:pubmed |
| pubmed-article:20331971 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20331971 | pubmed:day | 16 | lld:pubmed |
| pubmed-article:20331971 | pubmed:volume | 394 | lld:pubmed |
| pubmed-article:20331971 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20331971 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20331971 | pubmed:pagination | 1000-5 | lld:pubmed |
| pubmed-article:20331971 | pubmed:meshHeading | pubmed-meshheading:20331971... | lld:pubmed |
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| pubmed-article:20331971 | pubmed:meshHeading | pubmed-meshheading:20331971... | lld:pubmed |
| pubmed-article:20331971 | pubmed:meshHeading | pubmed-meshheading:20331971... | lld:pubmed |
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| pubmed-article:20331971 | pubmed:meshHeading | pubmed-meshheading:20331971... | lld:pubmed |
| pubmed-article:20331971 | pubmed:meshHeading | pubmed-meshheading:20331971... | lld:pubmed |
| pubmed-article:20331971 | pubmed:meshHeading | pubmed-meshheading:20331971... | lld:pubmed |
| pubmed-article:20331971 | pubmed:meshHeading | pubmed-meshheading:20331971... | lld:pubmed |
| pubmed-article:20331971 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20331971 | pubmed:articleTitle | Functional availability of gamma-herpesvirus K-cyclin is regulated by cellular CDK6 and p16INK4a. | lld:pubmed |
| pubmed-article:20331971 | pubmed:affiliation | Division of Chemotherapy, Faculty of Pharmacy, Keio University, 1-5-30 Shiba-koen, Minato-ku, Tokyo 105-8512, Japan. | lld:pubmed |
| pubmed-article:20331971 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20331971 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:20331971 | lld:entrezgene |
