| pubmed-article:20298757 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20298757 | lifeskim:mentions | umls-concept:C0038454 | lld:lifeskim |
| pubmed-article:20298757 | lifeskim:mentions | umls-concept:C0162638 | lld:lifeskim |
| pubmed-article:20298757 | lifeskim:mentions | umls-concept:C0521390 | lld:lifeskim |
| pubmed-article:20298757 | lifeskim:mentions | umls-concept:C0082529 | lld:lifeskim |
| pubmed-article:20298757 | lifeskim:mentions | umls-concept:C0165519 | lld:lifeskim |
| pubmed-article:20298757 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:20298757 | lifeskim:mentions | umls-concept:C1366765 | lld:lifeskim |
| pubmed-article:20298757 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:20298757 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:20298757 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:20298757 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:20298757 | pubmed:dateCreated | 2010-4-12 | lld:pubmed |
| pubmed-article:20298757 | pubmed:abstractText | Exercise preconditioning has been shown to reduce neuronal damage in ischemic/reperfusion (I/R) injury. ERK1/2 signaling in injury has been thought to modulate neuroprotection. In this study, we investigated the effects of ERK1/2 activation on the expression and activity of MMP-9 and downstream neuronal apoptosis. Adult male Sprague-Dawley rats were subjected to 30min of exercise on a treadmill for 3 weeks. Stroke was induced by a 2-h middle cerebral artery (MCA) occlusion using an intraluminal filament. Apoptotic protein caspase-3 and neuronal apoptosis in cortex and striatum was determined by Western blot at 24h reperfusion and TUNEL staining at 48h reperfusion in 5 I/R injury groups: no treatment, MMP-9 inhibitor (doxycycline), pre-ischemic exercise, exercised animals undergone ERK1/2 inhibition (U0126), and dual inhibition of ERK1/2 and MMP-9 in exercised ischemic rats. Cerebral MMP-9 expression in ischemic rats with different treatment was determined at 6, 12 and 24h reperfusion by real-time PCR for mRNA, Western blot for protein and zymography for enzyme activity. Exercise preconditioning significantly (p<0.05) reduced apoptosis determined by caspase-3 and TUNEL. In non-exercised rats, doxycycline treatment had significant (p<0.05) reductions in apoptosis after I/R injury. The dual ERK1/2-MMP-9 inhibited exercised animals had significantly (p<0.05) reduced neuronal apoptosis that was similar to that seen in exercised ischemic rats. MMP-9 expression in I/R injury was significantly (p<0.05) reduced in the exercised animals as compared to non-exercised controls. When ERK1/2 was inhibited, the reduced MMP-9 expression was reversed to the level seen in the non-exercised controls. This study has suggested that exercise-induced neuroprotection in I/R injury may be mediated by MMP-9 and ERK1/2 expression, leading to a reduction in neuronal apoptosis. | lld:pubmed |
| pubmed-article:20298757 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20298757 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20298757 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20298757 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20298757 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20298757 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20298757 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20298757 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20298757 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20298757 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20298757 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20298757 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20298757 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20298757 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:20298757 | pubmed:issn | 1872-7972 | lld:pubmed |
| pubmed-article:20298757 | pubmed:author | pubmed-author:GeeD ADA | lld:pubmed |
| pubmed-article:20298757 | pubmed:author | pubmed-author:OmidRR | lld:pubmed |
| pubmed-article:20298757 | pubmed:author | pubmed-author:DingYuchuanY | lld:pubmed |
| pubmed-article:20298757 | pubmed:author | pubmed-author:JimenezDavid... | lld:pubmed |
| pubmed-article:20298757 | pubmed:author | pubmed-author:JiXunmingX | lld:pubmed |
| pubmed-article:20298757 | pubmed:author | pubmed-author:GoelGunjanG | lld:pubmed |
| pubmed-article:20298757 | pubmed:author | pubmed-author:CarranzaAaron... | lld:pubmed |
| pubmed-article:20298757 | pubmed:author | pubmed-author:LiebeltBrando... | lld:pubmed |
| pubmed-article:20298757 | pubmed:author | pubmed-author:CurryAleciaA | lld:pubmed |
| pubmed-article:20298757 | pubmed:author | pubmed-author:ChaudhryKhawa... | lld:pubmed |
| pubmed-article:20298757 | pubmed:author | pubmed-author:RogersRyanR | lld:pubmed |
| pubmed-article:20298757 | pubmed:copyrightInfo | Copyright 2010 Elsevier Ireland Ltd. All rights reserved. | lld:pubmed |
| pubmed-article:20298757 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20298757 | pubmed:day | 26 | lld:pubmed |
| pubmed-article:20298757 | pubmed:volume | 474 | lld:pubmed |
| pubmed-article:20298757 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20298757 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20298757 | pubmed:pagination | 109-14 | lld:pubmed |
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| pubmed-article:20298757 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20298757 | pubmed:articleTitle | Matrix metalloproteinase-9 (MMP-9) expression and extracellular signal-regulated kinase 1 and 2 (ERK1/2) activation in exercise-reduced neuronal apoptosis after stroke. | lld:pubmed |
| pubmed-article:20298757 | pubmed:affiliation | Department of Neurological Surgery, Wayne State University School of Medicine, Detroit, MI 48201, United States. | lld:pubmed |
| pubmed-article:20298757 | pubmed:publicationType | Journal Article | lld:pubmed |
