| pubmed-article:20228121 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20228121 | lifeskim:mentions | umls-concept:C0001811 | lld:lifeskim |
| pubmed-article:20228121 | lifeskim:mentions | umls-concept:C0018787 | lld:lifeskim |
| pubmed-article:20228121 | lifeskim:mentions | umls-concept:C0225828 | lld:lifeskim |
| pubmed-article:20228121 | lifeskim:mentions | umls-concept:C0521451 | lld:lifeskim |
| pubmed-article:20228121 | lifeskim:mentions | umls-concept:C0215848 | lld:lifeskim |
| pubmed-article:20228121 | lifeskim:mentions | umls-concept:C0332161 | lld:lifeskim |
| pubmed-article:20228121 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:20228121 | lifeskim:mentions | umls-concept:C1704632 | lld:lifeskim |
| pubmed-article:20228121 | lifeskim:mentions | umls-concept:C0871261 | lld:lifeskim |
| pubmed-article:20228121 | lifeskim:mentions | umls-concept:C2911692 | lld:lifeskim |
| pubmed-article:20228121 | lifeskim:mentions | umls-concept:C1706817 | lld:lifeskim |
| pubmed-article:20228121 | lifeskim:mentions | umls-concept:C0392760 | lld:lifeskim |
| pubmed-article:20228121 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:20228121 | pubmed:dateCreated | 2010-5-20 | lld:pubmed |
| pubmed-article:20228121 | pubmed:abstractText | Ageing reduces the ability of cardiac myocytes to respond to inotropic agents. We hypothesized that hypoxia-inducible factor-1 (HIF-1) would improve the functional and Ca(2+) transient responses of ageing myocytes to the inotropic agents and this would act, in part, through altered mitochondrial activity. Young (3-4 months) and older Fischer 344 rats (18-20 months) were used. Hypoxia-inducible factor-1alpha was upregulated with ciclopirox olamine (CPX, 50 mg kg(1) on 2 days). Hypoxia-inducible factor-1 upregulation was detected by Western blot. Cardiomyocyte contraction and Ca(2+) transients were measured at baseline and after forskolin and ouabain. We also measured mitochondrial complex activities and production of reactive oxygen species (ROS). In the young group, forskolin (31%) and ouabain (31%) significantly increased percentage shortening. Similar changes were observed in the young + CPX group. Calcium transients also responded in a similar manner. However, in the older group, forskolin (12%) and ouabain (6%) did not significantly increase myocyte contractility or Ca(2+) transients. In the older + CPX group, the effects of forskolin (34%) and ouabain (29%) were restored. In the young + CPX group, there was increased ROS production and mitochondrial complex I and III activity compared with the young group. These differences were not observed in older groups. These data demonstrate an impaired functional and Ca(2+) effect of positive inotropic agents in older myocytes. Upregulation of HIF-1 restored this blunted response, but this was not related to changed mitochondrial activity induced by HIF-1. Thus, we found that HIF-1 improved inotropy in older myocytes without requiring mitochondrial activity changes. | lld:pubmed |
| pubmed-article:20228121 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20228121 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20228121 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20228121 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20228121 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20228121 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20228121 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20228121 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20228121 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20228121 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20228121 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20228121 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20228121 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20228121 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:20228121 | pubmed:issn | 1469-445X | lld:pubmed |
| pubmed-article:20228121 | pubmed:author | pubmed-author:TWW | lld:pubmed |
| pubmed-article:20228121 | pubmed:author | pubmed-author:WeissHarvey... | lld:pubmed |
| pubmed-article:20228121 | pubmed:author | pubmed-author:Marín-GarcíaJ... | lld:pubmed |
| pubmed-article:20228121 | pubmed:author | pubmed-author:DamleShirishS | lld:pubmed |
| pubmed-article:20228121 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20228121 | pubmed:volume | 95 | lld:pubmed |
| pubmed-article:20228121 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20228121 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20228121 | pubmed:pagination | 712-22 | lld:pubmed |
| pubmed-article:20228121 | pubmed:meshHeading | pubmed-meshheading:20228121... | lld:pubmed |
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| pubmed-article:20228121 | pubmed:meshHeading | pubmed-meshheading:20228121... | lld:pubmed |
| pubmed-article:20228121 | pubmed:meshHeading | pubmed-meshheading:20228121... | lld:pubmed |
| pubmed-article:20228121 | pubmed:meshHeading | pubmed-meshheading:20228121... | lld:pubmed |
| pubmed-article:20228121 | pubmed:meshHeading | pubmed-meshheading:20228121... | lld:pubmed |
| pubmed-article:20228121 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20228121 | pubmed:articleTitle | Hypoxia-inducible factor-1 improves inotropic responses of cardiac myocytes in ageing heart without affecting mitochondrial activity. | lld:pubmed |
| pubmed-article:20228121 | pubmed:affiliation | Department of Physiology and Biophysics, UMDNJ, Robert Wood Johnson Medical School, 675 Hoes Lane West, Piscataway, NJ 08854, USA. | lld:pubmed |
| pubmed-article:20228121 | pubmed:publicationType | Journal Article | lld:pubmed |
| entrez-gene:29560 | entrezgene:pubmed | pubmed-article:20228121 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:20228121 | lld:entrezgene |
