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pubmed-article:2021236pubmed:abstractTextExplants were prepared from skeletal muscle tissue from 5 nondystrophic pups and from 5 pups with X-linked muscular dystrophy; pups were 2 to 17 weeks old. A serial reexplant method resulted in optimal cell density with minimal fibroblast growth. Cultures were examined daily by use of phase-contrast microscopy; differentiated (post-fusion) cultures were examined by electron microscopy. Moderate nuclear pleomorphism and cell clustering were observed in cultures of normal and dystrophic muscle cells. Cultures were maintained to 27 days after plating. Minimal myofilament synthesis was observed in multinucleate cells from nondystrophic and dystrophic pups, but spontaneous contraction of myotubes was not observed during this period. Differences in growth, fusion, or differentiation of myogenic cells into multinucleate cells and myotubes were not found between dystrophic and normal muscle.lld:pubmed
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pubmed-article:2021236pubmed:authorpubmed-author:CooperB JBJlld:pubmed
pubmed-article:2021236pubmed:authorpubmed-author:CummingsJ FJFlld:pubmed
pubmed-article:2021236pubmed:authorpubmed-author:ValentineB...lld:pubmed
pubmed-article:2021236pubmed:authorpubmed-author:ChandlerS KSKlld:pubmed
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pubmed-article:2021236pubmed:volume52lld:pubmed
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pubmed-article:2021236pubmed:pagination104-7lld:pubmed
pubmed-article:2021236pubmed:dateRevised2010-11-18lld:pubmed
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pubmed-article:2021236pubmed:year1991lld:pubmed
pubmed-article:2021236pubmed:articleTitleIn vitro characteristics of normal and dystrophic skeletal muscle from dogs.lld:pubmed
pubmed-article:2021236pubmed:affiliationDepartment of Pathology, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853-6401.lld:pubmed
pubmed-article:2021236pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:2021236pubmed:publicationTypeComparative Studylld:pubmed
pubmed-article:2021236pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
pubmed-article:2021236pubmed:publicationTypeResearch Support, U.S. Gov't, Non-P.H.S.lld:pubmed
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