| pubmed-article:20171149 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20171149 | lifeskim:mentions | umls-concept:C2004454 | lld:lifeskim |
| pubmed-article:20171149 | lifeskim:mentions | umls-concept:C0012899 | lld:lifeskim |
| pubmed-article:20171149 | lifeskim:mentions | umls-concept:C1623415 | lld:lifeskim |
| pubmed-article:20171149 | lifeskim:mentions | umls-concept:C0098361 | lld:lifeskim |
| pubmed-article:20171149 | lifeskim:mentions | umls-concept:C0243072 | lld:lifeskim |
| pubmed-article:20171149 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:20171149 | pubmed:dateCreated | 2010-4-26 | lld:pubmed |
| pubmed-article:20171149 | pubmed:abstractText | Nucleotide excision repair (NER) removes the major UV-photolesions from cellular DNA. In humans, compromised NER activity is the cause of several photosensitive diseases, one of which is the skin-cancer predisposition disorder, xeroderma pigmentosum (XP). Two assays commonly used in measurement of NER activity are 'unscheduled DNA synthesis (UDS)', and 'recovery of RNA synthesis (RRS)', the latter being a specific measure of the transcription-coupled repair sub-pathway of NER. Both assays are key techniques for research in NER as well as in diagnoses of NER-related disorders. Until very recently, reliable methods for these assays involved measurements of incorporation of radio-labeled nucleosides. We have established non-radioactive procedures for determining UDS and RRS levels by incorporation of recently developed alkyne-conjugated nucleoside analogues, 5-ethynyl-2'-deoxyuridine (EdU) and 5-ethynyuridine (EU). EdU and EU are respectively used as alternatives for (3)H-thymidine in UDS and for (3)H-uridine in RRS. Based on these alkyne-nucleosides and an integrated image analyser, we have developed a semi-automated assay system for NER-activity. We demonstrate the utility of this system for NER-activity assessments of lymphoblastoid samples as well as primary fibroblasts. Potential use of the system for large-scale siRNA-screening for novel NER defects as well as for routine XP diagnosis are also considered. | lld:pubmed |
| pubmed-article:20171149 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20171149 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20171149 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20171149 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20171149 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20171149 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20171149 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20171149 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20171149 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20171149 | pubmed:month | May | lld:pubmed |
| pubmed-article:20171149 | pubmed:issn | 1568-7856 | lld:pubmed |
| pubmed-article:20171149 | pubmed:author | pubmed-author:LehmannAlan... | lld:pubmed |
| pubmed-article:20171149 | pubmed:author | pubmed-author:YamashitaShun... | lld:pubmed |
| pubmed-article:20171149 | pubmed:author | pubmed-author:OgiTomooT | lld:pubmed |
| pubmed-article:20171149 | pubmed:author | pubmed-author:NakazawaYukaY | lld:pubmed |
| pubmed-article:20171149 | pubmed:copyrightInfo | (c) 2010 Elsevier B.V. All rights reserved. | lld:pubmed |
| pubmed-article:20171149 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20171149 | pubmed:day | 4 | lld:pubmed |
| pubmed-article:20171149 | pubmed:volume | 9 | lld:pubmed |
| pubmed-article:20171149 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20171149 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20171149 | pubmed:pagination | 506-16 | lld:pubmed |
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| pubmed-article:20171149 | pubmed:meshHeading | pubmed-meshheading:20171149... | lld:pubmed |
| pubmed-article:20171149 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20171149 | pubmed:articleTitle | A semi-automated non-radioactive system for measuring recovery of RNA synthesis and unscheduled DNA synthesis using ethynyluracil derivatives. | lld:pubmed |
| pubmed-article:20171149 | pubmed:affiliation | Department of Molecular Medicine, Atomic Bomb Disease Institute, Graduate School of Biomedical Sciences, Nagasaki University, 1-12-4, Sakamoto, Nagasaki 852-8523, Japan. | lld:pubmed |
| pubmed-article:20171149 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20171149 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
