Comparison of the HIV-1 and HIV-2 proteinases using oligopeptide substrates representing cleavage sites in Gag and Gag-Pol polyproteins.

Source:http://linkedlifedata.com/resource/pubmed/id/2015912

FEBS Lett. 1991 Apr 9 281 1-2 77-80

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General Info

Authors

Oroszlan S, Copeland TD, Tözsér J, Bláha I, Wondrak EM

Affiliation

Laboratory of Molecular Virology and Carcinogenesis, ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, MD 21702.

Abstract

The substrate specificity of the human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2) proteinases was compared using oligopeptides corresponding to cleavage sites in the Gag and Gag-Pol polyproteins of both viruses. All peptides mimicking cleavage sites at the junction of major functional protein domains were correctly cleaved by both enzymes. However, some other peptides thought to represent secondary cleavage sites remained intact. The kinetic parameters (Km and kcat) obtained for the different substrates showed several hundred-fold variation but were similar for the same substrate.

PMID
2015912

Publication types

Comparative Study; Research Support, U.S. Gov't, P.H.S.