| pubmed-article:20152818 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20152818 | lifeskim:mentions | umls-concept:C0019704 | lld:lifeskim |
| pubmed-article:20152818 | lifeskim:mentions | umls-concept:C0001511 | lld:lifeskim |
| pubmed-article:20152818 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:20152818 | lifeskim:mentions | umls-concept:C0439640 | lld:lifeskim |
| pubmed-article:20152818 | lifeskim:mentions | umls-concept:C1539099 | lld:lifeskim |
| pubmed-article:20152818 | lifeskim:mentions | umls-concept:C1882417 | lld:lifeskim |
| pubmed-article:20152818 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:20152818 | pubmed:dateCreated | 2010-3-22 | lld:pubmed |
| pubmed-article:20152818 | pubmed:abstractText | The dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN) and DC-SIGN-related (DC-SIGNR) molecules on the cell surface are known to enhance human immunodeficiency virus type 1 (HIV-1) infection by capturing the virions and transmitting them to CD4+ T-cell, a process termed trans-infection. The neck region and carbohydrate recognition domain of the two proteins are important for efficient binding to the HIV-1 envelope protein. DC-SIGNR is polymorphic in Exons 4 and 5 that encode the neck region and carbohydrate recognition domain, respectively; the former contains a variable number of tandem repeats, and the latter the SNP (rs2277998). Since it remains unclear whether the DC-SIGNR polymorphism is related to the risk of HIV-1 infection, we tested possible effects of the polymorphism on HIV-1 trans-infection efficiency, by constructing six kinds of cDNAs encoding DC-SIGNR variants with various numbers of repeat units and various SNP. We were able to express the variants on the surface of Raji cells, a human B cell line. Flow cytometry showed that all the tested DC-SIGNR molecules were efficiently expressed on the cell surface at various levels; the assay for HIV trans-infection efficacy showed that all the tested variants had that activity with different efficacy levels. We found a correlation between the HIV trans-infection efficiency and the mean fluorescent intensity of DC-SIGNR expression (R(2)=0.95). Thus, our results suggest that the variation of the tested DC-SIGNR genotypes affects the efficacy of trans-infection by affecting the amounts of the protein expressed on the cell surface. | lld:pubmed |
| pubmed-article:20152818 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20152818 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20152818 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20152818 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20152818 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20152818 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20152818 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20152818 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20152818 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:20152818 | pubmed:issn | 1090-2104 | lld:pubmed |
| pubmed-article:20152818 | pubmed:author | pubmed-author:KitamuraYoshi... | lld:pubmed |
| pubmed-article:20152818 | pubmed:author | pubmed-author:IwamotoAikich... | lld:pubmed |
| pubmed-article:20152818 | pubmed:author | pubmed-author:Kawana-Tachik... | lld:pubmed |
| pubmed-article:20152818 | pubmed:author | pubmed-author:ZhuDayongD | lld:pubmed |
| pubmed-article:20152818 | pubmed:copyrightInfo | Copyright 2010 Elsevier Inc. All rights reserved. | lld:pubmed |
| pubmed-article:20152818 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20152818 | pubmed:day | 19 | lld:pubmed |
| pubmed-article:20152818 | pubmed:volume | 393 | lld:pubmed |
| pubmed-article:20152818 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20152818 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20152818 | pubmed:pagination | 598-602 | lld:pubmed |
| pubmed-article:20152818 | pubmed:meshHeading | pubmed-meshheading:20152818... | lld:pubmed |
| pubmed-article:20152818 | pubmed:meshHeading | pubmed-meshheading:20152818... | lld:pubmed |
| pubmed-article:20152818 | pubmed:meshHeading | pubmed-meshheading:20152818... | lld:pubmed |
| pubmed-article:20152818 | pubmed:meshHeading | pubmed-meshheading:20152818... | lld:pubmed |
| pubmed-article:20152818 | pubmed:meshHeading | pubmed-meshheading:20152818... | lld:pubmed |
| pubmed-article:20152818 | pubmed:meshHeading | pubmed-meshheading:20152818... | lld:pubmed |
| pubmed-article:20152818 | pubmed:meshHeading | pubmed-meshheading:20152818... | lld:pubmed |
| pubmed-article:20152818 | pubmed:meshHeading | pubmed-meshheading:20152818... | lld:pubmed |
| pubmed-article:20152818 | pubmed:meshHeading | pubmed-meshheading:20152818... | lld:pubmed |
| pubmed-article:20152818 | pubmed:meshHeading | pubmed-meshheading:20152818... | lld:pubmed |
| pubmed-article:20152818 | pubmed:meshHeading | pubmed-meshheading:20152818... | lld:pubmed |
| pubmed-article:20152818 | pubmed:meshHeading | pubmed-meshheading:20152818... | lld:pubmed |
| pubmed-article:20152818 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20152818 | pubmed:articleTitle | Influence of polymorphism in dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin-related (DC-SIGNR) gene on HIV-1 trans-infection. | lld:pubmed |
| pubmed-article:20152818 | pubmed:affiliation | Division of Infectious Diseases, Advanced Clinical Research Center, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan. zhu@ims.u-tokyo.ac.jp | lld:pubmed |
| pubmed-article:20152818 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20152818 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:10332 | entrezgene:pubmed | pubmed-article:20152818 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:20152818 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:20152818 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:20152818 | lld:entrezgene |
