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pubmed-article:20152438pubmed:abstractTextA novel fluorescence turn-on detection method of human serum albumin (HSA) and bovine serum albumin (BSA) in aqueous solution is investigated using 2,4-dihydroxyl-3-iodo salicylaldehyde azine (DISA). Upon the addition of DISA to HSA/BSA solution, a fluorescence turn-on effect at 529 nm can be observed with a large stokes shift of approximately 129 nm based on hydrophobic binding-mode between protein and dye. Under the optimal condition, the linear ranges of fluorescence intensity for HSA and BSA are 0.1-30 microg mL(-1) with the relative correlation coefficient of R(2)=0.991 (n=10) and 0.3-50 microg mL(-1) with R(2)=0.997 (n=10); and the detection limits for HSA and BSA based on IUPAC (C(DL)=3S(b)/m) are 20 ng mL(-1) and 50 ng mL(-1), respectively.lld:pubmed
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pubmed-article:20152438pubmed:authorpubmed-author:AlbazzazA NANlld:pubmed
pubmed-article:20152438pubmed:authorpubmed-author:YuXiangXlld:pubmed
pubmed-article:20152438pubmed:authorpubmed-author:TongAi-junAJlld:pubmed
pubmed-article:20152438pubmed:copyrightInfoCopyright (c) 2009 Elsevier B.V. All rights reserved.lld:pubmed
pubmed-article:20152438pubmed:issnTypeElectroniclld:pubmed
pubmed-article:20152438pubmed:day15lld:pubmed
pubmed-article:20152438pubmed:volume80lld:pubmed
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pubmed-article:20152438pubmed:pagination1952-8lld:pubmed
pubmed-article:20152438pubmed:dateRevised2010-11-18lld:pubmed
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pubmed-article:20152438pubmed:articleTitleFacile, sensitive and selective fluorescence turn-on detection of HSA/BSA in aqueous solution utilizing 2,4-dihydroxyl-3-iodo salicylaldehyde azine.lld:pubmed
pubmed-article:20152438pubmed:affiliationKey Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, PR China.lld:pubmed
pubmed-article:20152438pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:20152438pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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