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pubmed-article:20141131rdf:typepubmed:Citationlld:pubmed
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pubmed-article:20141131pubmed:issue8lld:pubmed
pubmed-article:20141131pubmed:dateCreated2010-2-24lld:pubmed
pubmed-article:20141131pubmed:abstractTextTotal synthesis and photophysical properties of PENB-DDAO, a photoactivatable 1,3-dichloro-9,9-dimethyl-9H-acridin-2(7)-one (DDAO) derivative of a far-red emitting fluorophore, are described. The photoremovable group of the DDAO phenolic function comprises a donor/acceptor biphenyl platform which allows an efficient (> or = 95%) and rapid (< 15 micros time-range) release of the fluorescent signal and displays remarkable two-photon uncaging cross sections (delta(a) x Phi(u) = 3.7 GM at 740 nm). PENB-DDAO is cell permeable as demonstrated by the triggering of cytoplasmic red fluorescent signal in HeLa cells after one-photon irradiation (lambda(exc) around 360 nm) or by the generation of a red fluorescent signal in a delineated area of a single cell after two-photon photoactivation (lambda(exc) = 770 nm).lld:pubmed
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pubmed-article:20141131pubmed:authorpubmed-author:VoneschJean-L...lld:pubmed
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pubmed-article:20141131pubmed:volume132lld:pubmed
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pubmed-article:20141131pubmed:articleTitleLive-cell one- and two-photon uncaging of a far-red emitting acridinone fluorophore.lld:pubmed
pubmed-article:20141131pubmed:affiliationLaboratoire de Conception et Application de Molécules Bioactives, UMR 7199 CNRS, Faculté de Pharmacie, Université de Strasbourg, 74 Route du Rhin 67401 Illkirch Cédex, France.lld:pubmed
pubmed-article:20141131pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:20141131pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed