| pubmed-article:20130102 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20130102 | lifeskim:mentions | umls-concept:C0242726 | lld:lifeskim |
| pubmed-article:20130102 | lifeskim:mentions | umls-concept:C0162741 | lld:lifeskim |
| pubmed-article:20130102 | lifeskim:mentions | umls-concept:C0040715 | lld:lifeskim |
| pubmed-article:20130102 | lifeskim:mentions | umls-concept:C0136993 | lld:lifeskim |
| pubmed-article:20130102 | lifeskim:mentions | umls-concept:C1710076 | lld:lifeskim |
| pubmed-article:20130102 | lifeskim:mentions | umls-concept:C1522702 | lld:lifeskim |
| pubmed-article:20130102 | lifeskim:mentions | umls-concept:C0392756 | lld:lifeskim |
| pubmed-article:20130102 | lifeskim:mentions | umls-concept:C0599718 | lld:lifeskim |
| pubmed-article:20130102 | lifeskim:mentions | umls-concept:C0599813 | lld:lifeskim |
| pubmed-article:20130102 | lifeskim:mentions | umls-concept:C0599893 | lld:lifeskim |
| pubmed-article:20130102 | lifeskim:mentions | umls-concept:C0052418 | lld:lifeskim |
| pubmed-article:20130102 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:20130102 | pubmed:dateCreated | 2010-4-6 | lld:pubmed |
| pubmed-article:20130102 | pubmed:abstractText | Complexation of arsenite [As(III)] with phytochelatins (PCs) is an important mechanism employed by plants to detoxify As; how this complexation affects As mobility was little known. We used high-resolution inductively coupled plasma-mass spectrometry and accurate mass electrospray ionization-mass spectrometry coupled to HPLC to identify and quantify As(III)-thiol complexes and free thiol compounds in Arabidopsis (Arabidopsis thaliana) exposed to arsenate [As(V)]. As(V) was efficiently reduced to As(III) in roots. In wild-type roots, 69% of As was complexed as As(III)-PC4, As(III)-PC3, and As(III)-(PC2)2. Both the glutathione (GSH)-deficient mutant cad2-1 and the PC-deficient mutant cad1-3 were approximately 20 times more sensitive to As(V) than the wild type. In cad1-3 roots, only 8% of As was complexed with GSH as As(III)-(GS)3 and no As(III)-PCs were detected, while in cad2-1 roots, As(III)-PCs accounted for only 25% of the total As. The two mutants had a greater As mobility, with a significantly higher accumulation of As(III) in shoots and 4.5 to 12 times higher shoot-to-root As concentration ratio than the wild type. Roots also effluxed a substantial proportion of the As(V) taken up as As(III) to the external medium, and this efflux was larger in the two mutants. Furthermore, when wild-type plants were exposed to l-buthionine sulfoximine or deprived of sulfur, both As(III) efflux and root-to-shoot translocation were enhanced. The results indicate that complexation of As(III) with PCs in Arabidopsis roots decreases its mobility for both efflux to the external medium and for root-to-shoot translocation. Enhancing PC synthesis in roots may be an effective strategy to reduce As translocation to the edible organs of food crops. | lld:pubmed |
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| pubmed-article:20130102 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20130102 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20130102 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:20130102 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20130102 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:20130102 | pubmed:issn | 1532-2548 | lld:pubmed |
| pubmed-article:20130102 | pubmed:author | pubmed-author:ZhaoFang-JieF... | lld:pubmed |
| pubmed-article:20130102 | pubmed:author | pubmed-author:McGrathSteve... | lld:pubmed |
| pubmed-article:20130102 | pubmed:author | pubmed-author:FeldmannJörgJ | lld:pubmed |
| pubmed-article:20130102 | pubmed:author | pubmed-author:RaabAndreaA | lld:pubmed |
| pubmed-article:20130102 | pubmed:author | pubmed-author:LiuWen-JuWJ | lld:pubmed |
| pubmed-article:20130102 | pubmed:author | pubmed-author:WoodB AlanBA | lld:pubmed |
| pubmed-article:20130102 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20130102 | pubmed:volume | 152 | lld:pubmed |
| pubmed-article:20130102 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20130102 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20130102 | pubmed:pagination | 2211-21 | lld:pubmed |
| pubmed-article:20130102 | pubmed:dateRevised | 2011-7-28 | lld:pubmed |
| pubmed-article:20130102 | pubmed:meshHeading | pubmed-meshheading:20130102... | lld:pubmed |
| pubmed-article:20130102 | pubmed:meshHeading | pubmed-meshheading:20130102... | lld:pubmed |
| pubmed-article:20130102 | pubmed:meshHeading | pubmed-meshheading:20130102... | lld:pubmed |
| pubmed-article:20130102 | pubmed:meshHeading | pubmed-meshheading:20130102... | lld:pubmed |
| pubmed-article:20130102 | pubmed:meshHeading | pubmed-meshheading:20130102... | lld:pubmed |
| pubmed-article:20130102 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20130102 | pubmed:articleTitle | Complexation of arsenite with phytochelatins reduces arsenite efflux and translocation from roots to shoots in Arabidopsis. | lld:pubmed |
| pubmed-article:20130102 | pubmed:affiliation | Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, United Kingdom. | lld:pubmed |
| pubmed-article:20130102 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20130102 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:20130102 | lld:pubmed |
