| pubmed-article:20112238 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20112238 | lifeskim:mentions | umls-concept:C0023764 | lld:lifeskim |
| pubmed-article:20112238 | lifeskim:mentions | umls-concept:C0243102 | lld:lifeskim |
| pubmed-article:20112238 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:20112238 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:20112238 | pubmed:dateCreated | 2010-5-17 | lld:pubmed |
| pubmed-article:20112238 | pubmed:abstractText | The effects of self-assembled polysaccharide nanogels on colloidal and thermal stability of lipase from Pseudomonas cepacia were investigated. The enzyme activity, especially k(cat), drastically increased in the presence of nanogels of cholesterol-bearing pullulan (CHP). The thermostability of lipase complex increased because the denaturation temperature of lipase increased by more than 20 degrees C by complexation with CHP nanogels. Lipase denaturation and aggregation upon heating was effectively prevented by complexation with CHP nanogels. Moreover, complexation with CHP nanogels protected lipase from lyophilization-induced aggregation. Nano-encapsulation with CHP nanogel is a useful method for colloidal and thermal stabilization of unstable enzyme. | lld:pubmed |
| pubmed-article:20112238 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20112238 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20112238 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20112238 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20112238 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20112238 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20112238 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20112238 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20112238 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20112238 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20112238 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:20112238 | pubmed:issn | 1616-5195 | lld:pubmed |
| pubmed-article:20112238 | pubmed:author | pubmed-author:AkiyoshiKazun... | lld:pubmed |
| pubmed-article:20112238 | pubmed:author | pubmed-author:SawadaShin-ic... | lld:pubmed |
| pubmed-article:20112238 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20112238 | pubmed:day | 8 | lld:pubmed |
| pubmed-article:20112238 | pubmed:volume | 10 | lld:pubmed |
| pubmed-article:20112238 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20112238 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20112238 | pubmed:pagination | 353-8 | lld:pubmed |
| pubmed-article:20112238 | pubmed:meshHeading | pubmed-meshheading:20112238... | lld:pubmed |
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| pubmed-article:20112238 | pubmed:meshHeading | pubmed-meshheading:20112238... | lld:pubmed |
| pubmed-article:20112238 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20112238 | pubmed:articleTitle | Nano-encapsulation of lipase by self-assembled nanogels: induction of high enzyme activity and thermal stabilization. | lld:pubmed |
| pubmed-article:20112238 | pubmed:affiliation | Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-surugadai, Chiyoda-ku, Tokyo 101-0062, Japan. | lld:pubmed |
| pubmed-article:20112238 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20112238 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
