| pubmed-article:20105429 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20105429 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:20105429 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:20105429 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:20105429 | lifeskim:mentions | umls-concept:C0025149 | lld:lifeskim |
| pubmed-article:20105429 | lifeskim:mentions | umls-concept:C0311400 | lld:lifeskim |
| pubmed-article:20105429 | lifeskim:mentions | umls-concept:C0376315 | lld:lifeskim |
| pubmed-article:20105429 | lifeskim:mentions | umls-concept:C0073096 | lld:lifeskim |
| pubmed-article:20105429 | lifeskim:mentions | umls-concept:C0449774 | lld:lifeskim |
| pubmed-article:20105429 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:20105429 | pubmed:dateCreated | 2010-3-9 | lld:pubmed |
| pubmed-article:20105429 | pubmed:abstractText | Cancer preventive reagent trans-resveratrol is intracellularly biotransformed to different metabolites. However, it is still unclear whether trans-resveratrol exerts its biological effects directly or through its metabolite(s). This issue was addressed here by identifying the metabolic pattern and the bioactive form of resveratrol in a resveratrol-sensitive human medulloblastoma cell line, UW228-3. The cell lysates and condition media of UW228-3 cells with or without 100 microM resveratrol treatment were analyzed by HPLC and LC/MS which revealed (1) that resveratrol was chemically unstable and the spontaneous generation of cis-resveratrol reduced resveratrol's anti-medulloblastoma efficacy and (2) that resveratrol monosulfate was the major metabolite of the cells. To identify the bioactive form of resveratrol, a mixture-containing approximately half fraction of resveratrol monosulfate was prepared by incubating trans-resveratrol with freshly prepared rat brain lysates. Medulloblastoma cells treated by 100 microM of this mixture showed attenuated cell crisis. The overall levels of the three brain-associated sulfotransferases (SULT1A1, 1C2 and 4A1) were low in medulloblastoma cells in vivo and in vitro in comparison with that in human noncancerous and rat normal cerebella; resveratrol could more or less up-regulate the production of these enzymes in UW228-3 cells but their overall level was still lower than that in normal cerebellum tissue. Our study thus demonstrated for the first time that trans-resveratrol is the bioactive form in medulloblastoma cells in which the expression of brain-associated SULTs was down-regulated, resulting in the increased intracellular bioavailability and anti-medulloblastoma efficacy of trans-resveratrol. | lld:pubmed |
| pubmed-article:20105429 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20105429 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20105429 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20105429 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20105429 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20105429 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20105429 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20105429 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20105429 | pubmed:month | May | lld:pubmed |
| pubmed-article:20105429 | pubmed:issn | 1873-2968 | lld:pubmed |
| pubmed-article:20105429 | pubmed:author | pubmed-author:LeeL JLJ | lld:pubmed |
| pubmed-article:20105429 | pubmed:author | pubmed-author:LiHongH | lld:pubmed |
| pubmed-article:20105429 | pubmed:author | pubmed-author:WangQianQ | lld:pubmed |
| pubmed-article:20105429 | pubmed:author | pubmed-author:SunYuanY | lld:pubmed |
| pubmed-article:20105429 | pubmed:author | pubmed-author:KongQing-YouQ... | lld:pubmed |
| pubmed-article:20105429 | pubmed:author | pubmed-author:ChenXiao-YanX... | lld:pubmed |
| pubmed-article:20105429 | pubmed:author | pubmed-author:SunZhengZ | lld:pubmed |
| pubmed-article:20105429 | pubmed:author | pubmed-author:WangJian-MinJ... | lld:pubmed |
| pubmed-article:20105429 | pubmed:author | pubmed-author:MaJing-XinJX | lld:pubmed |
| pubmed-article:20105429 | pubmed:author | pubmed-author:ShuXiao-HongX... | lld:pubmed |
| pubmed-article:20105429 | pubmed:author | pubmed-author:WuMo-LiML | lld:pubmed |
| pubmed-article:20105429 | pubmed:author | pubmed-author:FuYuan-ShanYS | lld:pubmed |
| pubmed-article:20105429 | pubmed:copyrightInfo | 2010 Elsevier Inc. All rights reserved. | lld:pubmed |
| pubmed-article:20105429 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20105429 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:20105429 | pubmed:volume | 79 | lld:pubmed |
| pubmed-article:20105429 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20105429 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20105429 | pubmed:pagination | 1516-25 | lld:pubmed |
| pubmed-article:20105429 | pubmed:meshHeading | pubmed-meshheading:20105429... | lld:pubmed |
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| pubmed-article:20105429 | pubmed:meshHeading | pubmed-meshheading:20105429... | lld:pubmed |
| pubmed-article:20105429 | pubmed:meshHeading | pubmed-meshheading:20105429... | lld:pubmed |
| pubmed-article:20105429 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20105429 | pubmed:articleTitle | Identification of metabolic pattern and bioactive form of resveratrol in human medulloblastoma cells. | lld:pubmed |
| pubmed-article:20105429 | pubmed:affiliation | Department of Cell Biology, Dalian Medical University, China. | lld:pubmed |
| pubmed-article:20105429 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20105429 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
