| pubmed-article:20018177 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20018177 | lifeskim:mentions | umls-concept:C0026848 | lld:lifeskim |
| pubmed-article:20018177 | lifeskim:mentions | umls-concept:C1704336 | lld:lifeskim |
| pubmed-article:20018177 | lifeskim:mentions | umls-concept:C2717940 | lld:lifeskim |
| pubmed-article:20018177 | lifeskim:mentions | umls-concept:C0074554 | lld:lifeskim |
| pubmed-article:20018177 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:20018177 | lifeskim:mentions | umls-concept:C1156530 | lld:lifeskim |
| pubmed-article:20018177 | lifeskim:mentions | umls-concept:C0686907 | lld:lifeskim |
| pubmed-article:20018177 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:20018177 | pubmed:dateCreated | 2010-2-17 | lld:pubmed |
| pubmed-article:20018177 | pubmed:abstractText | The mechanism of statin-induced skeletal muscle myopathy is poorly understood. We investigated how simvastatin affects cholesterol metabolism, ubiquinone levels, and the prenylation and N-linked glycosylation of proteins in C2C12 myotubes. We used liver HepG2 cells for comparison, as their responses to statins are well-characterized in terms of their cholesterol metabolism (in contrast to muscle cells), and statins are well-tolerated in the liver. Differences between the two cell lines could indicate the mechanism behind statin-induced myopathy. Simvastatin reduced de novo cholesterol production in C2C12 myotubes by 95% after 18h treatment. The reduction was 82% in the HepG2 cells. Total cholesterol pools, however, remained constant in both cell lines. Simvastatin treatment similarly did not affect total ubiquinone levels in the myotubes, unlike in HepG2 cells (22% reduction in CoQ10). Statin treatment reduced levels of Ras and Rap1 prenylation in both cell lines, whereas N-linked glycosylation was only affected in C2C12 myotubes (21% reduction in rate). From these observations, we conclude that total cholesterol and ubiquinone levels are unlikely to be involved in statin-mediated myopathy, but reductions in protein prenylation and especially N-linked glycosylation may play a role. This first comparison of the responses to simvastatin between liver and skeletal muscle cell lines may be important for future research directions concerning statin-induced myopathy. | lld:pubmed |
| pubmed-article:20018177 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20018177 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20018177 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20018177 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20018177 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20018177 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20018177 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20018177 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20018177 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20018177 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20018177 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20018177 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20018177 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:20018177 | pubmed:issn | 1873-2968 | lld:pubmed |
| pubmed-article:20018177 | pubmed:author | pubmed-author:KrähenbühlSte... | lld:pubmed |
| pubmed-article:20018177 | pubmed:author | pubmed-author:BrechtKarinK | lld:pubmed |
| pubmed-article:20018177 | pubmed:author | pubmed-author:ScharnaglHube... | lld:pubmed |
| pubmed-article:20018177 | pubmed:author | pubmed-author:MullenPeter... | lld:pubmed |
| pubmed-article:20018177 | pubmed:author | pubmed-author:LüscherBarbar... | lld:pubmed |
| pubmed-article:20018177 | pubmed:copyrightInfo | 2009 Elsevier Inc. All rights reserved. | lld:pubmed |
| pubmed-article:20018177 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20018177 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:20018177 | pubmed:volume | 79 | lld:pubmed |
| pubmed-article:20018177 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20018177 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20018177 | pubmed:pagination | 1200-9 | lld:pubmed |
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| pubmed-article:20018177 | pubmed:meshHeading | pubmed-meshheading:20018177... | lld:pubmed |
| pubmed-article:20018177 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20018177 | pubmed:articleTitle | Effect of simvastatin on cholesterol metabolism in C2C12 myotubes and HepG2 cells, and consequences for statin-induced myopathy. | lld:pubmed |
| pubmed-article:20018177 | pubmed:affiliation | Department of Research, University Hospital Basel, Switzerland. peter.mullen@unibas.ch | lld:pubmed |
| pubmed-article:20018177 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20018177 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
