| pubmed-article:20008810 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20008810 | lifeskim:mentions | umls-concept:C0041400 | lld:lifeskim |
| pubmed-article:20008810 | lifeskim:mentions | umls-concept:C0007589 | lld:lifeskim |
| pubmed-article:20008810 | lifeskim:mentions | umls-concept:C1135926 | lld:lifeskim |
| pubmed-article:20008810 | lifeskim:mentions | umls-concept:C0380603 | lld:lifeskim |
| pubmed-article:20008810 | lifeskim:mentions | umls-concept:C0231441 | lld:lifeskim |
| pubmed-article:20008810 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:20008810 | lifeskim:mentions | umls-concept:C1706284 | lld:lifeskim |
| pubmed-article:20008810 | lifeskim:mentions | umls-concept:C1524075 | lld:lifeskim |
| pubmed-article:20008810 | lifeskim:mentions | umls-concept:C2265852 | lld:lifeskim |
| pubmed-article:20008810 | lifeskim:mentions | umls-concept:C1511938 | lld:lifeskim |
| pubmed-article:20008810 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:20008810 | pubmed:dateCreated | 2009-12-17 | lld:pubmed |
| pubmed-article:20008810 | pubmed:abstractText | Glypican-1 is a cell membrane heparan sulfate proteoglycan that is composed of a core protein and covalently attached glycosaminoglycan (GAG) chains and N-linked glycosylated (N-glycosylated) chains. The glypican-1 GAG chains are required for cell differentiation and responsiveness to fibroblast growth factor 2 (FGF2). The role of glypican-1 N-glycosylated chains in regulating cell activities has not been reported. The objective of the current study was to investigate the role of glypican-1 N-glycosylated chains and the interaction between N-glycosylated and GAG chains in turkey myogenic satellite cell proliferation, differentiation, and FGF2 responsiveness. The wild-type turkey glypican-1 and turkey glypican-1 with mutated GAG chain attachment sites were cloned into the pCMS-EGFP mammalian expression vector and were used as templates to generate glypican-1 N-glycosylated 1-chain and no-chain mutants with or without GAG chains by site-directed mutagenesis. The wild-type glypican-1 and all glypican-1 N-glycosylated 1-chain and no-chain mutants with or without GAG chains were transfected into turkey myogenic satellite cells. Cell proliferation, differentiation, and FGF2 responsiveness were measured. The overexpression of glypican-1 N-glycosylated 1-chain and no-chain mutants without GAG chains increased cell proliferation and differentiation compared with the wild-type glypican-1 but not the glypican-1 N-glycosylated mutants with GAG chains attached. Cells overexpressing glypican-1 N-glycosylated mutants with or without GAG chains increased cell responsiveness to FGF2 compared with wild-type glypican-1. These data suggest that glypican-1 N-glycosylated chains and GAG chains are critical in regulating turkey myogenic satellite cell proliferation, differentiation, and responsivness to FGF2. | lld:pubmed |
| pubmed-article:20008810 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20008810 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20008810 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20008810 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20008810 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20008810 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20008810 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:20008810 | pubmed:issn | 0032-5791 | lld:pubmed |
| pubmed-article:20008810 | pubmed:author | pubmed-author:NestorK EKE | lld:pubmed |
| pubmed-article:20008810 | pubmed:author | pubmed-author:McFarlaneD... | lld:pubmed |
| pubmed-article:20008810 | pubmed:author | pubmed-author:SongYY | lld:pubmed |
| pubmed-article:20008810 | pubmed:author | pubmed-author:VellemanS GSG | lld:pubmed |
| pubmed-article:20008810 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:20008810 | pubmed:volume | 89 | lld:pubmed |
| pubmed-article:20008810 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20008810 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20008810 | pubmed:pagination | 123-34 | lld:pubmed |
| pubmed-article:20008810 | pubmed:meshHeading | pubmed-meshheading:20008810... | lld:pubmed |
| pubmed-article:20008810 | pubmed:meshHeading | pubmed-meshheading:20008810... | lld:pubmed |
| pubmed-article:20008810 | pubmed:meshHeading | pubmed-meshheading:20008810... | lld:pubmed |
| pubmed-article:20008810 | pubmed:meshHeading | pubmed-meshheading:20008810... | lld:pubmed |
| pubmed-article:20008810 | pubmed:meshHeading | pubmed-meshheading:20008810... | lld:pubmed |
| pubmed-article:20008810 | pubmed:meshHeading | pubmed-meshheading:20008810... | lld:pubmed |
| pubmed-article:20008810 | pubmed:meshHeading | pubmed-meshheading:20008810... | lld:pubmed |
| pubmed-article:20008810 | pubmed:meshHeading | pubmed-meshheading:20008810... | lld:pubmed |
| pubmed-article:20008810 | pubmed:meshHeading | pubmed-meshheading:20008810... | lld:pubmed |
| pubmed-article:20008810 | pubmed:meshHeading | pubmed-meshheading:20008810... | lld:pubmed |
| pubmed-article:20008810 | pubmed:meshHeading | pubmed-meshheading:20008810... | lld:pubmed |
| pubmed-article:20008810 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20008810 | pubmed:articleTitle | Effect of glypican-1 covalently attached chains on turkey myogenic satellite cell proliferation, differentiation, and fibroblast growth factor 2 responsiveness. | lld:pubmed |
| pubmed-article:20008810 | pubmed:affiliation | Department of Animal Sciences, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster 44691, USA. | lld:pubmed |
| pubmed-article:20008810 | pubmed:publicationType | Journal Article | lld:pubmed |
| entrez-gene:693243 | entrezgene:pubmed | pubmed-article:20008810 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:20008810 | lld:entrezgene |
