Linked Life Data

19956942

Source:http://linkedlifedata.com/resource/pubmed/id/19956942

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:19956942rdf:typepubmed:Citationlld:pubmed
pubmed-article:19956942lifeskim:mentionsumls-concept:C0315191lld:lifeskim
pubmed-article:19956942lifeskim:mentionsumls-concept:C0033684lld:lifeskim
pubmed-article:19956942lifeskim:mentionsumls-concept:C0220781lld:lifeskim
pubmed-article:19956942lifeskim:mentionsumls-concept:C0220839lld:lifeskim
pubmed-article:19956942lifeskim:mentionsumls-concept:C1883254lld:lifeskim
pubmed-article:19956942lifeskim:mentionsumls-concept:C0033268lld:lifeskim
pubmed-article:19956942lifeskim:mentionsumls-concept:C1515568lld:lifeskim
pubmed-article:19956942lifeskim:mentionsumls-concept:C1514873lld:lifeskim
pubmed-article:19956942pubmed:issue3lld:pubmed
pubmed-article:19956942pubmed:dateCreated2010-3-18lld:pubmed
pubmed-article:19956942pubmed:abstractTextWe found that penicillin-induced glutamate production by Corynebacterium glutamicum is inhibited when a de novo protein synthesis inhibitor, chloramphenicol, is added simultaneously with penicillin. When chloramphenicol was added 4 h after penicillin addition, glutamate production was essentially unaffected. (3)H-Leucine incorporation experiments revealed that protein synthesis continued for 1 h after penicillin addition and then gradually decreased. These results suggest that de novo protein synthesis within 4 h of penicillin treatment is required for the induction of glutamate production. To identify the protein(s) necessary for penicillin-induced glutamate production, proteome analysis of penicillin-treated C. glutamicum cells was performed with two-dimensional gel electrophoresis. Of more than 500 proteins detected, the amount of 13 proteins, including OdhI (an inhibitory protein for 2-oxoglutarate dehydrogenase complex), significantly increased upon penicillin treatment. Artificial overexpression of the odhI gene resulted in the decreased specific activity of the 2-oxoglutarate dehydrogenase complex and increased glutamate production without any triggers. These results suggest that the de novo synthesis of OdhI is the necessary factor for penicillin-induced glutamate overproduction by C. glutamicum. Moreover, continuous glutamate production was achieved by overexpression of odhI without any triggers. Thus, the odhI-overexpressing strain of C. glutamicum can be useful for efficient glutamate production.lld:pubmed
pubmed-article:19956942pubmed:languageenglld:pubmed
pubmed-article:19956942pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19956942pubmed:citationSubsetIMlld:pubmed
pubmed-article:19956942pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19956942pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19956942pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19956942pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19956942pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19956942pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19956942pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19956942pubmed:statusMEDLINElld:pubmed
pubmed-article:19956942pubmed:monthAprlld:pubmed
pubmed-article:19956942pubmed:issn1432-0614lld:pubmed
pubmed-article:19956942pubmed:authorpubmed-author:WachiMasaakiMlld:pubmed
pubmed-article:19956942pubmed:authorpubmed-author:NagaiKazuoKlld:pubmed
pubmed-article:19956942pubmed:authorpubmed-author:ShimizuHirosh...lld:pubmed
pubmed-article:19956942pubmed:authorpubmed-author:HirasawaTakas...lld:pubmed
pubmed-article:19956942pubmed:authorpubmed-author:NagahisaKeisu...lld:pubmed
pubmed-article:19956942pubmed:authorpubmed-author:KimJongpillJlld:pubmed
pubmed-article:19956942pubmed:authorpubmed-author:FukudaHirohis...lld:pubmed
pubmed-article:19956942pubmed:issnTypeElectroniclld:pubmed
pubmed-article:19956942pubmed:volume86lld:pubmed
pubmed-article:19956942pubmed:ownerNLMlld:pubmed
pubmed-article:19956942pubmed:authorsCompleteYlld:pubmed
pubmed-article:19956942pubmed:pagination911-20lld:pubmed
pubmed-article:19956942pubmed:meshHeadingpubmed-meshheading:19956942...lld:pubmed
pubmed-article:19956942pubmed:meshHeadingpubmed-meshheading:19956942...lld:pubmed
pubmed-article:19956942pubmed:meshHeadingpubmed-meshheading:19956942...lld:pubmed
pubmed-article:19956942pubmed:meshHeadingpubmed-meshheading:19956942...lld:pubmed
pubmed-article:19956942pubmed:meshHeadingpubmed-meshheading:19956942...lld:pubmed
pubmed-article:19956942pubmed:meshHeadingpubmed-meshheading:19956942...lld:pubmed
pubmed-article:19956942pubmed:meshHeadingpubmed-meshheading:19956942...lld:pubmed
pubmed-article:19956942pubmed:meshHeadingpubmed-meshheading:19956942...lld:pubmed
pubmed-article:19956942pubmed:meshHeadingpubmed-meshheading:19956942...lld:pubmed
pubmed-article:19956942pubmed:meshHeadingpubmed-meshheading:19956942...lld:pubmed
pubmed-article:19956942pubmed:meshHeadingpubmed-meshheading:19956942...lld:pubmed
pubmed-article:19956942pubmed:meshHeadingpubmed-meshheading:19956942...lld:pubmed
pubmed-article:19956942pubmed:year2010lld:pubmed
pubmed-article:19956942pubmed:articleTitleRequirement of de novo synthesis of the OdhI protein in penicillin-induced glutamate production by Corynebacterium glutamicum.lld:pubmed
pubmed-article:19956942pubmed:affiliationDepartment of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan.lld:pubmed
pubmed-article:19956942pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:19956942pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
http://linkedlifedata.com/r...entrezgene:pubmedpubmed-article:19956942lld:entrezgene