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pubmed-article:19933263pubmed:abstractTextThe supramolecular assembly of a novel cyanine dye, 3,3'-di(3-sulfopropyl)-4,5,4',5'-dibenzo-9-ethyl-thiacarbocyanine triethylammonium salt (ETC) was designed to verify specific intramolecular G-quadruplexes from duplex and single-strand DNAs. Spectral results have shown that ETC presented two major distinct signatures with specific intramolecular G-quadruplexes in vitro: (i) dramatic changes in the absorption spectra (including disappearance of absorption peak around 660 nm and appearance of independent new peak around 584 nm); (ii) approximately 70 times enhancement of fluorescence signal at 600 nm. Furthermore, based on (1)H-nuclear magnetic resonance and circular dichroism results, the preferring binding of ETC to specific intramolecular G-quadruplexes probably result from end-stacking, and the loop structure nearby also plays an important role.lld:pubmed
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pubmed-article:19933263pubmed:authorpubmed-author:OmuroHHlld:pubmed
pubmed-article:19933263pubmed:authorpubmed-author:ZhangHongHlld:pubmed
pubmed-article:19933263pubmed:authorpubmed-author:LiQianQlld:pubmed
pubmed-article:19933263pubmed:authorpubmed-author:YangShuSlld:pubmed
pubmed-article:19933263pubmed:authorpubmed-author:XiangJunfengJlld:pubmed
pubmed-article:19933263pubmed:authorpubmed-author:XuGuangzhiGlld:pubmed
pubmed-article:19933263pubmed:authorpubmed-author:TangYalinYlld:pubmed
pubmed-article:19933263pubmed:authorpubmed-author:ZhangXiufengXlld:pubmed
pubmed-article:19933263pubmed:authorpubmed-author:YangQianfanQlld:pubmed
pubmed-article:19933263pubmed:authorpubmed-author:GuanAijiaoAlld:pubmed
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pubmed-article:19933263pubmed:dateRevised2010-9-28lld:pubmed
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pubmed-article:19933263pubmed:articleTitleVerification of specific G-quadruplex structure by using a novel cyanine dye supramolecular assembly: II. The binding characterization with specific intramolecular G-quadruplex and the recognizing mechanism.lld:pubmed
pubmed-article:19933263pubmed:affiliationInstitute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.lld:pubmed
pubmed-article:19933263pubmed:publicationTypeJournal Articlelld:pubmed