| pubmed-article:19931239 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19931239 | lifeskim:mentions | umls-concept:C0009368 | lld:lifeskim |
| pubmed-article:19931239 | lifeskim:mentions | umls-concept:C0031671 | lld:lifeskim |
| pubmed-article:19931239 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:19931239 | lifeskim:mentions | umls-concept:C0591833 | lld:lifeskim |
| pubmed-article:19931239 | pubmed:issue | 1-3 | lld:pubmed |
| pubmed-article:19931239 | pubmed:dateCreated | 2010-1-27 | lld:pubmed |
| pubmed-article:19931239 | pubmed:abstractText | The muscarinic receptor subtype M(3) is coupled to Gq/11 proteins. Muscarinic receptor agonists such as carbachol stimulate these receptors that result in activation of phospholipase C (PLC) which hydrolyzes phosphatidylinositol 4,5-bisphosphate into diacylglycerol and Ins(1,4,5)P(3). This pathway leads to excitation and smooth muscle contraction. In this study the PLC agonist, 2, 4, 6-trimethyl-N-(meta-3-trifluoromethyl-phenyl)-benezenesulfonamide (m-3M3FBS), was used to investigate whether direct PLC activation mimics carbachol-induced excitation. We examined the effects of m-3M3FBS and 2, 4, 6-trimethyl-N-(ortho-3-trifluoromethyl-phenyl)-benzenesulfonamide (o-3M3FBS), on murine colonic smooth muscle tissue and cells by performing conventional microelectrode recordings, isometric force measurements and patch clamp experiments. Application of m-3M3FBS decreased spontaneous contractility in murine colonic smooth muscle without affecting the resting membrane potential. Patch clamp studies revealed that delayed rectifier K(+) channels were reversibly inhibited by m-3M3FBS and o-3M3FBS. The PLC inhibitor, 1-(6-((17b-3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione (U73122), did not prevent this inhibition by m-3M3FBS. Both m-3M3FBS and o-3M3FBS decreased two components of delayed rectifier K(+) currents in the presence of tetraethylammonium chloride or 4-aminopyridine. Ca(2+) currents were significantly suppressed by m-3M3FBS and o-3M3FBS with a simultaneous increase in intracellular Ca(2+). Pretreatment with U73122 did not prevent the decrease in Ca(2+) currents upon m-3M3FBS application. In conclusion, both m-3M3FBS and o-3M3FBS inhibit inward and outward currents via mechanisms independent of PLC acting in an antagonistic manner. In contrast, both compounds also caused an increase in [Ca(2+)](i) in an agonistic manner. Therefore caution must be employed when interpreting their effects at the tissue and cellular level. | lld:pubmed |
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| pubmed-article:19931239 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19931239 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19931239 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19931239 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:19931239 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19931239 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:19931239 | pubmed:issn | 1879-0712 | lld:pubmed |
| pubmed-article:19931239 | pubmed:author | pubmed-author:KohSang DonSD | lld:pubmed |
| pubmed-article:19931239 | pubmed:author | pubmed-author:KimHyun JinHJ | lld:pubmed |
| pubmed-article:19931239 | pubmed:author | pubmed-author:DwyerLauraL | lld:pubmed |
| pubmed-article:19931239 | pubmed:author | pubmed-author:KohByoung... | lld:pubmed |
| pubmed-article:19931239 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19931239 | pubmed:day | 25 | lld:pubmed |
| pubmed-article:19931239 | pubmed:volume | 628 | lld:pubmed |
| pubmed-article:19931239 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19931239 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19931239 | pubmed:pagination | 187-94 | lld:pubmed |
| pubmed-article:19931239 | pubmed:dateRevised | 2011-8-25 | lld:pubmed |
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| pubmed-article:19931239 | pubmed:meshHeading | pubmed-meshheading:19931239... | lld:pubmed |
| pubmed-article:19931239 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:19931239 | pubmed:articleTitle | Phospholipase C-independent effects of 3M3FBS in murine colon. | lld:pubmed |
| pubmed-article:19931239 | pubmed:affiliation | Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, Nevada 89557, USA. | lld:pubmed |
| pubmed-article:19931239 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19931239 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
