| pubmed-article:19927117 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19927117 | lifeskim:mentions | umls-concept:C0025255 | lld:lifeskim |
| pubmed-article:19927117 | lifeskim:mentions | umls-concept:C0205147 | lld:lifeskim |
| pubmed-article:19927117 | lifeskim:mentions | umls-concept:C0023779 | lld:lifeskim |
| pubmed-article:19927117 | lifeskim:mentions | umls-concept:C1412504 | lld:lifeskim |
| pubmed-article:19927117 | lifeskim:mentions | umls-concept:C1423774 | lld:lifeskim |
| pubmed-article:19927117 | lifeskim:mentions | umls-concept:C0812409 | lld:lifeskim |
| pubmed-article:19927117 | lifeskim:mentions | umls-concept:C0549178 | lld:lifeskim |
| pubmed-article:19927117 | lifeskim:mentions | umls-concept:C0205134 | lld:lifeskim |
| pubmed-article:19927117 | lifeskim:mentions | umls-concept:C0205324 | lld:lifeskim |
| pubmed-article:19927117 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:19927117 | pubmed:dateCreated | 2010-1-20 | lld:pubmed |
| pubmed-article:19927117 | pubmed:abstractText | ArfGAP1, which promotes GTP hydrolysis on the small G protein Arf1 on Golgi membranes, interacts preferentially with positively curved membranes through its amphipathic lipid packing sensor (ALPS) motifs. This should influence the distribution of Arf1-GTP when flat and curved regions coexist on a continuous membrane, notably during COPI vesicle budding. To test this, we pulled tubes from giant vesicles using molecular motors or optical tweezers. Arf1-GTP distributed on the giant vesicles and on the tubes, whereas ArfGAP1 bound exclusively to the tubes. Decreasing the tube radius revealed a threshold of R approximately 35 nm for the binding of ArfGAP1 ALPS motifs. Mixing catalytic amounts of ArfGAP1 with Arf1-GTP induced a smooth Arf1 gradient along the tube. This reflects that Arf1 molecules leaving the tube on GTP hydrolysis are replaced by new Arf1-GTP molecules diffusing from the giant vesicle. The characteristic length of the gradient is two orders of magnitude larger than a COPI bud, suggesting that Arf1-GTP diffusion can readily compensate for the localized loss of Arf1 during budding and contribute to the stability of the coat until fission. | lld:pubmed |
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| pubmed-article:19927117 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19927117 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19927117 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19927117 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:19927117 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19927117 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:19927117 | pubmed:issn | 1460-2075 | lld:pubmed |
| pubmed-article:19927117 | pubmed:author | pubmed-author:GoudBrunoB | lld:pubmed |
| pubmed-article:19927117 | pubmed:author | pubmed-author:BassereauPatr... | lld:pubmed |
| pubmed-article:19927117 | pubmed:author | pubmed-author:MannevilleJea... | lld:pubmed |
| pubmed-article:19927117 | pubmed:author | pubmed-author:AntonnyBrunoB | lld:pubmed |
| pubmed-article:19927117 | pubmed:author | pubmed-author:AmbroggioErne... | lld:pubmed |
| pubmed-article:19927117 | pubmed:author | pubmed-author:SorreBenoîtB | lld:pubmed |
| pubmed-article:19927117 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19927117 | pubmed:day | 20 | lld:pubmed |
| pubmed-article:19927117 | pubmed:volume | 29 | lld:pubmed |
| pubmed-article:19927117 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19927117 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19927117 | pubmed:pagination | 292-303 | lld:pubmed |
| pubmed-article:19927117 | pubmed:dateRevised | 2011-7-20 | lld:pubmed |
| pubmed-article:19927117 | pubmed:meshHeading | pubmed-meshheading:19927117... | lld:pubmed |
| pubmed-article:19927117 | pubmed:meshHeading | pubmed-meshheading:19927117... | lld:pubmed |
| pubmed-article:19927117 | pubmed:meshHeading | pubmed-meshheading:19927117... | lld:pubmed |
| pubmed-article:19927117 | pubmed:meshHeading | pubmed-meshheading:19927117... | lld:pubmed |
| pubmed-article:19927117 | pubmed:meshHeading | pubmed-meshheading:19927117... | lld:pubmed |
| pubmed-article:19927117 | pubmed:meshHeading | pubmed-meshheading:19927117... | lld:pubmed |
| pubmed-article:19927117 | pubmed:meshHeading | pubmed-meshheading:19927117... | lld:pubmed |
| pubmed-article:19927117 | pubmed:meshHeading | pubmed-meshheading:19927117... | lld:pubmed |
| pubmed-article:19927117 | pubmed:meshHeading | pubmed-meshheading:19927117... | lld:pubmed |
| pubmed-article:19927117 | pubmed:meshHeading | pubmed-meshheading:19927117... | lld:pubmed |
| pubmed-article:19927117 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:19927117 | pubmed:articleTitle | ArfGAP1 generates an Arf1 gradient on continuous lipid membranes displaying flat and curved regions. | lld:pubmed |
| pubmed-article:19927117 | pubmed:affiliation | Laboratoire Mécanismes moléculaires du transport intracellulaire, Institut Curie, CNRS UMR 144, Paris, France. | lld:pubmed |
| pubmed-article:19927117 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19927117 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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