| pubmed-article:19910685 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19910685 | lifeskim:mentions | umls-concept:C0030274 | lld:lifeskim |
| pubmed-article:19910685 | lifeskim:mentions | umls-concept:C1704675 | lld:lifeskim |
| pubmed-article:19910685 | lifeskim:mentions | umls-concept:C1424878 | lld:lifeskim |
| pubmed-article:19910685 | lifeskim:mentions | umls-concept:C0128479 | lld:lifeskim |
| pubmed-article:19910685 | lifeskim:mentions | umls-concept:C1256369 | lld:lifeskim |
| pubmed-article:19910685 | pubmed:issue | 5-6 | lld:pubmed |
| pubmed-article:19910685 | pubmed:dateCreated | 2009-11-13 | lld:pubmed |
| pubmed-article:19910685 | pubmed:abstractText | Apolipoprotein A-V is an important determinant of plasma triglyceride level in both humans and mice. This study showed the physiological impact of apoA-V on insulin secretion in rat pancreatic beta-cells (INS-1 cells). In order to precise the mechanism of action, binding experiments coupled to mass spectrometry were performed to identify a potential membrane receptor. Results showed an interaction between apoA-V and midkine protein. Confocal microscopy confirmed the plasma membrane co-localisation of this two-proteins after the treatment of INS-1 cells with the apo-AV recombinant protein and indicated that the cell surface midkine could be involved in apoA-V endocytosis, since these two proteins were co-translocated at the plasma membrane or in the cytosol compartment. This co-localisation is correlated with an increase in insulin secretion in a dose dependant manner during short incubation period. Reduction of midkine expression by small interfering RNA duplexes revealed a decrease in the ability of these transfected cells to secrete insulin in presence of apoA-V. Competition experiments for the apoA-V-midkine binding at the cell surface using antibody directed against midkine is able to influence INS-1 cell function as insulin secretion. Our results showed apoA-V ability to enhance insulin secretion in beta-cells and provide evidence of an internalization pathway involving the midkine as partner. | lld:pubmed |
| pubmed-article:19910685 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19910685 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19910685 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19910685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19910685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19910685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19910685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19910685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19910685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19910685 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19910685 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19910685 | pubmed:issn | 1421-9778 | lld:pubmed |
| pubmed-article:19910685 | pubmed:author | pubmed-author:FruchartJean-... | lld:pubmed |
| pubmed-article:19910685 | pubmed:author | pubmed-author:DrobecqHervéH | lld:pubmed |
| pubmed-article:19910685 | pubmed:author | pubmed-author:Fruchart-Naji... | lld:pubmed |
| pubmed-article:19910685 | pubmed:author | pubmed-author:NowakMaximeM | lld:pubmed |
| pubmed-article:19910685 | pubmed:author | pubmed-author:RommensCorinn... | lld:pubmed |
| pubmed-article:19910685 | pubmed:author | pubmed-author:DehondtHélène... | lld:pubmed |
| pubmed-article:19910685 | pubmed:author | pubmed-author:HelleboidStép... | lld:pubmed |
| pubmed-article:19910685 | pubmed:author | pubmed-author:Helleboid-Cha... | lld:pubmed |
| pubmed-article:19910685 | pubmed:author | pubmed-author:HéliotLaurent... | lld:pubmed |
| pubmed-article:19910685 | pubmed:author | pubmed-author:MoitrotEmmanu... | lld:pubmed |
| pubmed-article:19910685 | pubmed:copyrightInfo | 2009 S. Karger AG, Basel. | lld:pubmed |
| pubmed-article:19910685 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19910685 | pubmed:volume | 24 | lld:pubmed |
| pubmed-article:19910685 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19910685 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19910685 | pubmed:pagination | 451-60 | lld:pubmed |
| pubmed-article:19910685 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:19910685 | pubmed:meshHeading | pubmed-meshheading:19910685... | lld:pubmed |
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| pubmed-article:19910685 | pubmed:meshHeading | pubmed-meshheading:19910685... | lld:pubmed |
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| pubmed-article:19910685 | pubmed:meshHeading | pubmed-meshheading:19910685... | lld:pubmed |
| pubmed-article:19910685 | pubmed:meshHeading | pubmed-meshheading:19910685... | lld:pubmed |
| pubmed-article:19910685 | pubmed:meshHeading | pubmed-meshheading:19910685... | lld:pubmed |
| pubmed-article:19910685 | pubmed:meshHeading | pubmed-meshheading:19910685... | lld:pubmed |
| pubmed-article:19910685 | pubmed:meshHeading | pubmed-meshheading:19910685... | lld:pubmed |
| pubmed-article:19910685 | pubmed:meshHeading | pubmed-meshheading:19910685... | lld:pubmed |
| pubmed-article:19910685 | pubmed:meshHeading | pubmed-meshheading:19910685... | lld:pubmed |
| pubmed-article:19910685 | pubmed:meshHeading | pubmed-meshheading:19910685... | lld:pubmed |
| pubmed-article:19910685 | pubmed:meshHeading | pubmed-meshheading:19910685... | lld:pubmed |
| pubmed-article:19910685 | pubmed:meshHeading | pubmed-meshheading:19910685... | lld:pubmed |
| pubmed-article:19910685 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19910685 | pubmed:articleTitle | Apolipoprotein A-V modulates insulin secretion in pancreatic beta-cells through its interaction with midkine. | lld:pubmed |
| pubmed-article:19910685 | pubmed:affiliation | Université Lille Nord de France, Inserm, UDSL and Institut Pasteur de Lille, France. audrey.helleboid@univ-lille2.fr | lld:pubmed |
| pubmed-article:19910685 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19910685 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:19910685 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:19910685 | lld:entrezgene |
