| pubmed-article:19881308 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19881308 | lifeskim:mentions | umls-concept:C0007586 | lld:lifeskim |
| pubmed-article:19881308 | lifeskim:mentions | umls-concept:C2936320 | lld:lifeskim |
| pubmed-article:19881308 | lifeskim:mentions | umls-concept:C1521827 | lld:lifeskim |
| pubmed-article:19881308 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:19881308 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:19881308 | lifeskim:mentions | umls-concept:C0441712 | lld:lifeskim |
| pubmed-article:19881308 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:19881308 | pubmed:dateCreated | 2009-11-2 | lld:pubmed |
| pubmed-article:19881308 | pubmed:abstractText | Control of cell cycle progression in somatic cells or terminally differentiated cells is a key technology for cell-based therapies such as regenerative therapy. We have prepared an artificial cell cycle progression peptide composed of a human immunodeficiency virus-derived Tat protein transduction domain (PTD) and a p53 genetic suppressor element (GSE123). The peptide significantly promoted hepatocyte growth factor-induced DNA synthesis and the proliferation of primary mouse hepatocytes, which are highly differentiated somatic cells. The addition of a nuclear localization signal (NLS) sequence to the peptide increased the internalization of the peptide to the nuclear fraction. Distribution analysis using a fluoresein isothiocyanate-labeled peptide indicated that the NLS enabled the peptide to escape from the lysosomes to the cytosol. As a result, the NLS-Tat-GSE123 peptide induced potent cell proliferation of primary mouse hepatocytes in vitro. The use of this peptide as an artificial cell growth enhancer, bypassing a specific receptor, is a useful tool for the study of regenerative therapy and cell signaling. | lld:pubmed |
| pubmed-article:19881308 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19881308 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19881308 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19881308 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19881308 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19881308 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19881308 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19881308 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19881308 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19881308 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:19881308 | pubmed:issn | 1347-5215 | lld:pubmed |
| pubmed-article:19881308 | pubmed:author | pubmed-author:WatanabeYoshi... | lld:pubmed |
| pubmed-article:19881308 | pubmed:author | pubmed-author:KankiKeitaK | lld:pubmed |
| pubmed-article:19881308 | pubmed:author | pubmed-author:IshijimaYukoY | lld:pubmed |
| pubmed-article:19881308 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19881308 | pubmed:volume | 32 | lld:pubmed |
| pubmed-article:19881308 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19881308 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19881308 | pubmed:pagination | 1917-20 | lld:pubmed |
| pubmed-article:19881308 | pubmed:meshHeading | pubmed-meshheading:19881308... | lld:pubmed |
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| pubmed-article:19881308 | pubmed:meshHeading | pubmed-meshheading:19881308... | lld:pubmed |
| pubmed-article:19881308 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19881308 | pubmed:articleTitle | Preparation of an artificial cell cycle progressor with a novel mechanism. | lld:pubmed |
| pubmed-article:19881308 | pubmed:affiliation | Department of Pharmaceutical Sciences, Musashino University, 1-1-20 Shinmachi, Nishitokyo, Tokyo 202-8585, Japan. | lld:pubmed |
| pubmed-article:19881308 | pubmed:publicationType | Journal Article | lld:pubmed |
