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pubmed-article:19851034pubmed:abstractTextThe purpose of these studies was to explore the expression pattern of miRNAs associated with invasion and metastasis of human papillary thyroid carcinoma (PTC). A transwell invasion chamber was used to select progressively more invasive cancer cell populations from a clonal cell line of human PTC with cervical lymph node metastasis, IHH-4. Three sublines with progressive invasiveness, designated IHH-4M-1, IHH-4M-2 and IHH-4M-3, were obtained through this in vitro selection process. The sublines manifested an increase in colony-forming ability on soft agar and metastatic potency in nude mice. Then metastasis-related miRNAs differentially expressed between them were analyzed utilizing the miRNA microarray technique. We found that 11 metastasis-related miRNAs were differentially expressed between the invasive subpopulations and their control subpopulations; these miRNAs may account for their significant difference in the invasion capabilities and favor lymphatic metastasis of PTC.lld:pubmed
pubmed-article:19851034pubmed:languageenglld:pubmed
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pubmed-article:19851034pubmed:authorpubmed-author:WangHongHlld:pubmed
pubmed-article:19851034pubmed:authorpubmed-author:ZhangHongmeiHlld:pubmed
pubmed-article:19851034pubmed:authorpubmed-author:GaoYunYlld:pubmed
pubmed-article:19851034pubmed:authorpubmed-author:TengWeipingWlld:pubmed
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pubmed-article:19851034pubmed:authorpubmed-author:GuanHaixiaHlld:pubmed
pubmed-article:19851034pubmed:authorpubmed-author:FanChenlingClld:pubmed
pubmed-article:19851034pubmed:authorpubmed-author:MaoJinyuanJlld:pubmed
pubmed-article:19851034pubmed:authorpubmed-author:WangCuifangClld:pubmed
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pubmed-article:19851034pubmed:volume57lld:pubmed
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pubmed-article:19851034pubmed:pagination81-6lld:pubmed
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pubmed-article:19851034pubmed:year2010lld:pubmed
pubmed-article:19851034pubmed:articleTitlemiRNA expression in a human papillary thyroid carcinoma cell line varies with invasiveness.lld:pubmed
pubmed-article:19851034pubmed:affiliationEndocrine Department and Institute of Endocrinology, First Affiliated Hospital of China Medical University, Shenyang, China.lld:pubmed
pubmed-article:19851034pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:19851034pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed