| pubmed-article:19830445 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19830445 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:19830445 | lifeskim:mentions | umls-concept:C0007452 | lld:lifeskim |
| pubmed-article:19830445 | lifeskim:mentions | umls-concept:C0014597 | lld:lifeskim |
| pubmed-article:19830445 | lifeskim:mentions | umls-concept:C0021853 | lld:lifeskim |
| pubmed-article:19830445 | lifeskim:mentions | umls-concept:C0443211 | lld:lifeskim |
| pubmed-article:19830445 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:19830445 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:19830445 | pubmed:dateCreated | 2010-1-21 | lld:pubmed |
| pubmed-article:19830445 | pubmed:abstractText | Membranous epithelial cells (M cells) of the follicle-associated epithelium in Peyer's patches have a high capacity for transcytosis of several viruses and microorganisms. Here, we report that we have successfully established a bovine intestinal epithelial cell line (BIE cells) and developed an in vitro M cell model. BIE cells have a cobblestone morphology and microvilli-like structures, and strongly express cell-to-cell junctional proteins and cytokeratin, which is a specific intermediate filament protein of epithelial cells. After co-culture with murine intestinal lymphocytes or treatment with supernatant from bovine PBMC cultured with IL-2, BIE cells acquired the ability of transcytosis. Therefore, BIE cells have typical characteristics of bovine intestinal epithelial cells and also have the ability to differentiate into an M cell like linage. In addition, our results indicate that contact between immune cells and epithelial cells may not be absolutely required for the differentiation of M cells. We think that BIE cells will be useful for studying the transport mechanisms of various pathogens and also the evaluation of drug delivery via M cells. | lld:pubmed |
| pubmed-article:19830445 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19830445 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19830445 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19830445 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19830445 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:19830445 | pubmed:issn | 1432-119X | lld:pubmed |
| pubmed-article:19830445 | pubmed:author | pubmed-author:YamaguchiTaka... | lld:pubmed |
| pubmed-article:19830445 | pubmed:author | pubmed-author:AsoHisashiH | lld:pubmed |
| pubmed-article:19830445 | pubmed:author | pubmed-author:KitazawaHaruk... | lld:pubmed |
| pubmed-article:19830445 | pubmed:author | pubmed-author:RoseMichael... | lld:pubmed |
| pubmed-article:19830445 | pubmed:author | pubmed-author:MiyazawaKohta... | lld:pubmed |
| pubmed-article:19830445 | pubmed:author | pubmed-author:KanayaTakashi... | lld:pubmed |
| pubmed-article:19830445 | pubmed:author | pubmed-author:TanakaSachiS | lld:pubmed |
| pubmed-article:19830445 | pubmed:author | pubmed-author:TakakuraIkuro... | lld:pubmed |
| pubmed-article:19830445 | pubmed:author | pubmed-author:ItaniWataruW | lld:pubmed |
| pubmed-article:19830445 | pubmed:author | pubmed-author:HondoTetsuyaT | lld:pubmed |
| pubmed-article:19830445 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19830445 | pubmed:volume | 133 | lld:pubmed |
| pubmed-article:19830445 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19830445 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19830445 | pubmed:pagination | 125-34 | lld:pubmed |
| pubmed-article:19830445 | pubmed:meshHeading | pubmed-meshheading:19830445... | lld:pubmed |
| pubmed-article:19830445 | pubmed:meshHeading | pubmed-meshheading:19830445... | lld:pubmed |
| pubmed-article:19830445 | pubmed:meshHeading | pubmed-meshheading:19830445... | lld:pubmed |
| pubmed-article:19830445 | pubmed:meshHeading | pubmed-meshheading:19830445... | lld:pubmed |
| pubmed-article:19830445 | pubmed:meshHeading | pubmed-meshheading:19830445... | lld:pubmed |
| pubmed-article:19830445 | pubmed:meshHeading | pubmed-meshheading:19830445... | lld:pubmed |
| pubmed-article:19830445 | pubmed:meshHeading | pubmed-meshheading:19830445... | lld:pubmed |
| pubmed-article:19830445 | pubmed:meshHeading | pubmed-meshheading:19830445... | lld:pubmed |
| pubmed-article:19830445 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:19830445 | pubmed:articleTitle | Characterization of newly established bovine intestinal epithelial cell line. | lld:pubmed |
| pubmed-article:19830445 | pubmed:affiliation | Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai, Japan. kohtaroh.miyazawa@yale.edu | lld:pubmed |
| pubmed-article:19830445 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19830445 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
