| pubmed-article:1982480 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1982480 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:1982480 | lifeskim:mentions | umls-concept:C0025936 | lld:lifeskim |
| pubmed-article:1982480 | lifeskim:mentions | umls-concept:C0521390 | lld:lifeskim |
| pubmed-article:1982480 | lifeskim:mentions | umls-concept:C0038999 | lld:lifeskim |
| pubmed-article:1982480 | lifeskim:mentions | umls-concept:C0600688 | lld:lifeskim |
| pubmed-article:1982480 | lifeskim:mentions | umls-concept:C0599946 | lld:lifeskim |
| pubmed-article:1982480 | pubmed:dateCreated | 1991-5-24 | lld:pubmed |
| pubmed-article:1982480 | pubmed:abstractText | The role of oxygen-derived free radicals, superoxide in particular, in the pathogenesis of neuronal cell death induced by glutamate was studied using primary culture cortical neurons from transgenic mice overexpressing human CuZn-superoxide dismutase. Primary cortical neuron cultures were developed form 15-day-old fetuses of both transgenic mice and their normal littermates. Both human CuZn-superoxide dismutase and host mouse CuZn-superoxide dismutase activities in cultured neurons were identified by native gel electrophoresis followed by nitroblue tetrazolium staining. Cultured neurons grown for 10-12 days in vitro were exposed briefly to 0.5 mM glutamate for 5 minutes, followed by biochemical and morphological examinations at 2 and 4 hours. Our data have demonstrated that glutamate neurotoxicity is significantly reduced in transgenic neurons at 2 and 4 hours following exposure to glutamate, as measured by the intracellular 3-0-methyl glucose space, the efflux of lactate dehydrogenase, and by phase-contrast and bright-field trypan blue staining. These data indicate that transgenic neurons containing two- to threefold the normal amount of CuZn-superoxide dismutase activity are protected against glutamate neurotoxicity in vitro. Our results suggest that oxidative stress play an important role in glutamate-induced neuronal swelling and toxicity. | lld:pubmed |
| pubmed-article:1982480 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1982480 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1982480 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1982480 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1982480 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1982480 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1982480 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1982480 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1982480 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1982480 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1982480 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1982480 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1982480 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1982480 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1982480 | pubmed:issn | 0065-1419 | lld:pubmed |
| pubmed-article:1982480 | pubmed:author | pubmed-author:ChanP HPH | lld:pubmed |
| pubmed-article:1982480 | pubmed:author | pubmed-author:EpsteinC JCJ | lld:pubmed |
| pubmed-article:1982480 | pubmed:author | pubmed-author:CarlsonE JEJ | lld:pubmed |
| pubmed-article:1982480 | pubmed:author | pubmed-author:ChuLL | lld:pubmed |
| pubmed-article:1982480 | pubmed:author | pubmed-author:ChenS FSF | lld:pubmed |
| pubmed-article:1982480 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1982480 | pubmed:volume | 51 | lld:pubmed |
| pubmed-article:1982480 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1982480 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1982480 | pubmed:pagination | 245-7 | lld:pubmed |
| pubmed-article:1982480 | pubmed:dateRevised | 2009-11-11 | lld:pubmed |
| pubmed-article:1982480 | pubmed:meshHeading | pubmed-meshheading:1982480-... | lld:pubmed |
| pubmed-article:1982480 | pubmed:meshHeading | pubmed-meshheading:1982480-... | lld:pubmed |
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| pubmed-article:1982480 | pubmed:meshHeading | pubmed-meshheading:1982480-... | lld:pubmed |
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| pubmed-article:1982480 | pubmed:meshHeading | pubmed-meshheading:1982480-... | lld:pubmed |
| pubmed-article:1982480 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:1982480 | pubmed:articleTitle | Attenuation of glutamate-induced neuronal swelling and toxicity in transgenic mice overexpressing human CuZn-superoxide dismutase. | lld:pubmed |
| pubmed-article:1982480 | pubmed:affiliation | Department of Neurosurgery, University of California School of Medicine, San Francisco. | lld:pubmed |
| pubmed-article:1982480 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1982480 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
