| pubmed-article:19822763 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19822763 | lifeskim:mentions | umls-concept:C0003315 | lld:lifeskim |
| pubmed-article:19822763 | lifeskim:mentions | umls-concept:C0039194 | lld:lifeskim |
| pubmed-article:19822763 | lifeskim:mentions | umls-concept:C1704675 | lld:lifeskim |
| pubmed-article:19822763 | lifeskim:mentions | umls-concept:C0597545 | lld:lifeskim |
| pubmed-article:19822763 | lifeskim:mentions | umls-concept:C0444706 | lld:lifeskim |
| pubmed-article:19822763 | lifeskim:mentions | umls-concept:C0439662 | lld:lifeskim |
| pubmed-article:19822763 | lifeskim:mentions | umls-concept:C0441722 | lld:lifeskim |
| pubmed-article:19822763 | lifeskim:mentions | umls-concept:C0242849 | lld:lifeskim |
| pubmed-article:19822763 | pubmed:issue | 42 | lld:pubmed |
| pubmed-article:19822763 | pubmed:dateCreated | 2009-10-21 | lld:pubmed |
| pubmed-article:19822763 | pubmed:abstractText | During adaptive immune responses, T lymphocytes recognize antigenic peptides presented by MHC molecules on antigen-presenting cells (APCs). This recognition results in the formation of a so-called immune synapse (IS) at the T-cell/APC interface, which is crucial for T-cell activation. The molecular composition of the IS has been extensively studied, but little is known about the biophysics and interaction forces between T cells and APCs. Here, we report the measurement of interaction forces between T cells and APCs employing atomic force microscopy (AFM). For these investigations, specific T cells were selected that recognize an antigenic peptide presented by MHC-class II molecules on APCs. Dynamic analysis of T-cell/APC interaction by AFM revealed that in the presence of antigen interaction forces increased from 1 to 2 nN at early time-points to a maximum of approximately 14 nN after 30 min and decreased again after 60 min. These data correlate with the kinetics of synapse formation that also reached a maximum after 30 min, as determined by high-throughput multispectral imaging flow cytometry. Because the integrin lymphocyte function antigen-1 (LFA-1) and its counterpart intercellular adhesion molecule-1 (ICAM-1) are prominent members of a mature IS, the effect of a small molecular inhibitor for LFA-1, BIRT377, was investigated. BIRT377 almost completely abolish the interaction forces, emphasizing the importance of LFA-1/ICAM-1-interactions for firm T-cell/APC adhesion. In conclusion, using biophysical measurements, this study provides precise values for the interaction forces between T cells and APCs and demonstrates that these forces develop over time and are highest when synapse formation is maximal. | lld:pubmed |
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| pubmed-article:19822763 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19822763 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19822763 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19822763 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:19822763 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19822763 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:19822763 | pubmed:issn | 1091-6490 | lld:pubmed |
| pubmed-article:19822763 | pubmed:author | pubmed-author:HämmerlingGün... | lld:pubmed |
| pubmed-article:19822763 | pubmed:author | pubmed-author:BulbucNadjaN | lld:pubmed |
| pubmed-article:19822763 | pubmed:author | pubmed-author:SpatzJoachim... | lld:pubmed |
| pubmed-article:19822763 | pubmed:author | pubmed-author:GunzerMatthia... | lld:pubmed |
| pubmed-article:19822763 | pubmed:author | pubmed-author:SamstagYvonne... | lld:pubmed |
| pubmed-article:19822763 | pubmed:author | pubmed-author:WabnitzGuido... | lld:pubmed |
| pubmed-article:19822763 | pubmed:author | pubmed-author:DjandjiDomini... | lld:pubmed |
| pubmed-article:19822763 | pubmed:author | pubmed-author:LoubanIliaI | lld:pubmed |
| pubmed-article:19822763 | pubmed:author | pubmed-author:HosseiniBabak... | lld:pubmed |
| pubmed-article:19822763 | pubmed:author | pubmed-author:DeegJanoschJ | lld:pubmed |
| pubmed-article:19822763 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19822763 | pubmed:day | 20 | lld:pubmed |
| pubmed-article:19822763 | pubmed:volume | 106 | lld:pubmed |
| pubmed-article:19822763 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19822763 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19822763 | pubmed:pagination | 17852-7 | lld:pubmed |
| pubmed-article:19822763 | pubmed:dateRevised | 2010-9-27 | lld:pubmed |
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| pubmed-article:19822763 | pubmed:meshHeading | pubmed-meshheading:19822763... | lld:pubmed |
| pubmed-article:19822763 | pubmed:meshHeading | pubmed-meshheading:19822763... | lld:pubmed |
| pubmed-article:19822763 | pubmed:meshHeading | pubmed-meshheading:19822763... | lld:pubmed |
| pubmed-article:19822763 | pubmed:meshHeading | pubmed-meshheading:19822763... | lld:pubmed |
| pubmed-article:19822763 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19822763 | pubmed:articleTitle | Immune synapse formation determines interaction forces between T cells and antigen-presenting cells measured by atomic force microscopy. | lld:pubmed |
| pubmed-article:19822763 | pubmed:affiliation | Department of New Materials and Biosystems, Max-Planck-Institute for Metals Research, Stuttgart, Germany. | lld:pubmed |
| pubmed-article:19822763 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19822763 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:19822763 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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