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| pubmed-article:19786595 | lifeskim:mentions | umls-concept:C0009316 | lld:lifeskim |
| pubmed-article:19786595 | lifeskim:mentions | umls-concept:C0542341 | lld:lifeskim |
| pubmed-article:19786595 | lifeskim:mentions | umls-concept:C1704632 | lld:lifeskim |
| pubmed-article:19786595 | lifeskim:mentions | umls-concept:C0871261 | lld:lifeskim |
| pubmed-article:19786595 | lifeskim:mentions | umls-concept:C0018270 | lld:lifeskim |
| pubmed-article:19786595 | lifeskim:mentions | umls-concept:C1261322 | lld:lifeskim |
| pubmed-article:19786595 | lifeskim:mentions | umls-concept:C2911692 | lld:lifeskim |
| pubmed-article:19786595 | lifeskim:mentions | umls-concept:C1706817 | lld:lifeskim |
| pubmed-article:19786595 | lifeskim:mentions | umls-concept:C0205390 | lld:lifeskim |
| pubmed-article:19786595 | lifeskim:mentions | umls-concept:C0242849 | lld:lifeskim |
| pubmed-article:19786595 | lifeskim:mentions | umls-concept:C1522605 | lld:lifeskim |
| pubmed-article:19786595 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:19786595 | pubmed:dateCreated | 2009-11-17 | lld:pubmed |
| pubmed-article:19786595 | pubmed:abstractText | The prevalence of infections caused by multidrug-resistant gram-negative Acinetobacter baumannii strains and the lack of novel antibiotics under development are posing a global dilemma, forcing a resurgence of the last-line antibiotic colistin. Our aim was to use atomic force microscopy (AFM) to investigate the morphology and topography of paired colistin-susceptible and -resistant cells from colistin-heteroresistant A. baumannii strains as a function of bacterial growth phase and colistin exposure. An optimal AFM bacterial sample preparation protocol was established and applied to examine three paired strains. Images revealed rod-shaped colistin-susceptible cells (1.65 +/- 0.27 microm by 0.98 +/- 0.07 microm) at mid-logarithmic phase, in contrast to spherical colistin-resistant cells (1.03 +/- 0.09 microm); the latter were also more diverse in appearance and exhibited a rougher surface topography (7.05 +/- 1.3 nm versus 11.4 +/- 2.5 nm for susceptible versus resistant, respectively). Cellular elongation up to approximately 18 microm at stationary phase was more commonly observed in susceptible strains, although these "worm-like" cells were also observed occasionally in the resistant population. The effects of colistin exposure on the cell surface of colistin-susceptible and -resistant cells were found to be similar; topographical changes were minor in response to 0.5 microg/ml colistin; however, at 4 microg/ml colistin, a significant degree of surface disruption was detected. At 32 microg/ml colistin, cellular clumping and surface smoothening were evident. Our study has demonstrated for the first time substantial morphological and topographical differences between colistin-susceptible and -resistant cells from heteroresistant A. baumannii strains. These results contribute to an understanding of colistin action and resistance in regard to this problematic pathogen. | lld:pubmed |
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| pubmed-article:19786595 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19786595 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19786595 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:19786595 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19786595 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:19786595 | pubmed:issn | 1098-6596 | lld:pubmed |
| pubmed-article:19786595 | pubmed:author | pubmed-author:LiJianJ | lld:pubmed |
| pubmed-article:19786595 | pubmed:author | pubmed-author:NationRoger... | lld:pubmed |
| pubmed-article:19786595 | pubmed:author | pubmed-author:LarsonIanI | lld:pubmed |
| pubmed-article:19786595 | pubmed:author | pubmed-author:HartleyPatric... | lld:pubmed |
| pubmed-article:19786595 | pubmed:author | pubmed-author:SoonRachel... | lld:pubmed |
| pubmed-article:19786595 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19786595 | pubmed:volume | 53 | lld:pubmed |
| pubmed-article:19786595 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19786595 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19786595 | pubmed:pagination | 4979-86 | lld:pubmed |
| pubmed-article:19786595 | pubmed:dateRevised | 2011-8-1 | lld:pubmed |
| pubmed-article:19786595 | pubmed:meshHeading | pubmed-meshheading:19786595... | lld:pubmed |
| pubmed-article:19786595 | pubmed:meshHeading | pubmed-meshheading:19786595... | lld:pubmed |
| pubmed-article:19786595 | pubmed:meshHeading | pubmed-meshheading:19786595... | lld:pubmed |
| pubmed-article:19786595 | pubmed:meshHeading | pubmed-meshheading:19786595... | lld:pubmed |
| pubmed-article:19786595 | pubmed:meshHeading | pubmed-meshheading:19786595... | lld:pubmed |
| pubmed-article:19786595 | pubmed:meshHeading | pubmed-meshheading:19786595... | lld:pubmed |
| pubmed-article:19786595 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19786595 | pubmed:articleTitle | Atomic force microscopy investigation of the morphology and topography of colistin-heteroresistant Acinetobacter baumannii strains as a function of growth phase and in response to colistin treatment. | lld:pubmed |
| pubmed-article:19786595 | pubmed:affiliation | Facility for Anti-Infective Drug Development and Innovation, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, Victoria 3052, Australia. | lld:pubmed |
| pubmed-article:19786595 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19786595 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:19786595 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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