| pubmed-article:1977836 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1977836 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:1977836 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:1977836 | lifeskim:mentions | umls-concept:C0225336 | lld:lifeskim |
| pubmed-article:1977836 | lifeskim:mentions | umls-concept:C0026473 | lld:lifeskim |
| pubmed-article:1977836 | lifeskim:mentions | umls-concept:C0001511 | lld:lifeskim |
| pubmed-article:1977836 | lifeskim:mentions | umls-concept:C0597357 | lld:lifeskim |
| pubmed-article:1977836 | lifeskim:mentions | umls-concept:C0439064 | lld:lifeskim |
| pubmed-article:1977836 | lifeskim:mentions | umls-concept:C1314939 | lld:lifeskim |
| pubmed-article:1977836 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:1977836 | pubmed:dateCreated | 1990-12-4 | lld:pubmed |
| pubmed-article:1977836 | pubmed:abstractText | Monocytes exhibit significant basal (unstimulated) adherence to human umbilical vein endothelium (HUVE), which is only partially inhibited by an anti-CD18 monoclonal antibody (mAb) (60.3). We examined factors modulating the residual, CD18-independent monocyte binding to HUVE by pretreating monocytes with mAb 60.3 to eliminate CD18-dependent binding. Basal adherence was reduced from 32% +/- 2% to 14% +/- 2% with mAb 60.3 (means +/- SE of eight experiments; P less than 0.01). mAb 60.3-treated monocytes were incubated with tumor necrosis factor-gamma (TNF-alpha), interleukin-1 (IL-1), lipopolysaccharide (LPS), N-formylmethionyl-leucyl-phenylalamine (FMLP), or phorbol myristate acetate (PMA). Only PMA affected CD18-independent binding. Pretreatment with PMA alone reduced adherence to 21% +/- 2% (mean +/- SE of eight experiments; P less than 0.01). In conjunction with mAb 60.3, PMA virtually eliminated monocyte adherence to HUVE (7% +/- 1%, mean +/- SE of eight experiments; P less than 0.01). We also examined CD18-independent monocyte binding to endothelial-leukocyte adhesion molecules (E-LAMs) induced by pretreatment of HUVE with LPS. Monoclonal antibody 60.3-treated monocytes increased adherence from 14% +/- 2% with unstimulated HUVE to 37% +/- 2% with LPS-stimulated HUVE (mean +/- SE of four experiments; P less than 0.01). Monocytes pretreated with both mAb 60.3 and PMA increased adherence from 5% +/- 1% with the unstimulated HUVE to 18% +/- 1% with the LPS-stimulated HUVE (mean +/- SE of four experiments; P less than 0.01). This result implies the presence of a CD18-independent and PMA-insensitive receptor on human monocytes for an E-LAM induced by LPS. In summary, we have identified two CD18-independent mechanisms of monocyte adherence to HUVE; a PMA-sensitive mechanism mediating basal adherence and a PMA-insensitive mechanism involved in binding to E-LAMs. | lld:pubmed |
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| pubmed-article:1977836 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1977836 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1977836 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1977836 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1977836 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:1977836 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1977836 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1977836 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:1977836 | pubmed:issn | 0741-5400 | lld:pubmed |
| pubmed-article:1977836 | pubmed:author | pubmed-author:RossRR | lld:pubmed |
| pubmed-article:1977836 | pubmed:author | pubmed-author:HarlanJ MJM | lld:pubmed |
| pubmed-article:1977836 | pubmed:author | pubmed-author:DobrinaAA | lld:pubmed |
| pubmed-article:1977836 | pubmed:author | pubmed-author:CarlosT MTM | lld:pubmed |
| pubmed-article:1977836 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1977836 | pubmed:volume | 48 | lld:pubmed |
| pubmed-article:1977836 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1977836 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1977836 | pubmed:pagination | 451-6 | lld:pubmed |
| pubmed-article:1977836 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:1977836 | pubmed:meshHeading | pubmed-meshheading:1977836-... | lld:pubmed |
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| pubmed-article:1977836 | pubmed:meshHeading | pubmed-meshheading:1977836-... | lld:pubmed |
| pubmed-article:1977836 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:1977836 | pubmed:articleTitle | Multiple receptors on human monocytes are involved in adhesion to cultured human endothelial cells. | lld:pubmed |
| pubmed-article:1977836 | pubmed:affiliation | Department of Medicine, University of Washington, Seattle. | lld:pubmed |
| pubmed-article:1977836 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1977836 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1977836 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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