| pubmed-article:19771552 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19771552 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:19771552 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:19771552 | lifeskim:mentions | umls-concept:C2698187 | lld:lifeskim |
| pubmed-article:19771552 | lifeskim:mentions | umls-concept:C0812413 | lld:lifeskim |
| pubmed-article:19771552 | lifeskim:mentions | umls-concept:C0205396 | lld:lifeskim |
| pubmed-article:19771552 | lifeskim:mentions | umls-concept:C0887824 | lld:lifeskim |
| pubmed-article:19771552 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:19771552 | pubmed:issue | 22 | lld:pubmed |
| pubmed-article:19771552 | pubmed:dateCreated | 2009-11-25 | lld:pubmed |
| pubmed-article:19771552 | pubmed:abstractText | We performed proteomic differential display analysis of human malignant pleural mesothelioma (MPM) cell lines and a human pleural mesothelial cell line by using 2-DE and LC-MS/MS. The human MPM cell lines were NCI-H28, NCI-H2052 and NCI-H2452, and the human pleural mesothelial cell line was MeT-5A. Between MeT-5A and NCI-H2052, we found 38 protein spots whose expression levels were different, from the results of 2-DE; 28 protein spots appeared higher, and 10 other protein spots lower in NCI-H2052 than in MeT-5A. These spots were analyzed by LC-MS/MS analysis and identified by a peptide sequence tag. However, from the results of 2-DE of the other cell lines, there was only one consistently upregulated protein, astrocytic phosphoprotein PEA-15, in all three MPM cell lines. Western blotting using specific antibodies against PEA-15 confirmed the elevated expression level of PEA-15 in all three MPM cell lines compared with MeT-5A cells and normal pleura tissues from patients. PEA-15 was knocked down in NCI-H2052 cells, and the proliferation of PEA-15-silenced NCI-H2052 cells was suppressed 7-15% compared with negative control cells. These results suggest that PEA-15 expression is likely to be associated with the tumorigenesis of MPM. | lld:pubmed |
| pubmed-article:19771552 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19771552 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19771552 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19771552 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19771552 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19771552 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19771552 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19771552 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:19771552 | pubmed:issn | 1615-9861 | lld:pubmed |
| pubmed-article:19771552 | pubmed:author | pubmed-author:TanakaToshiki... | lld:pubmed |
| pubmed-article:19771552 | pubmed:author | pubmed-author:TanakaToshiyu... | lld:pubmed |
| pubmed-article:19771552 | pubmed:author | pubmed-author:HamanoKimikaz... | lld:pubmed |
| pubmed-article:19771552 | pubmed:author | pubmed-author:NakamuraKazuy... | lld:pubmed |
| pubmed-article:19771552 | pubmed:author | pubmed-author:KuramitsuYasu... | lld:pubmed |
| pubmed-article:19771552 | pubmed:author | pubmed-author:FujimotoMasan... | lld:pubmed |
| pubmed-article:19771552 | pubmed:author | pubmed-author:UedaKazuhiroK | lld:pubmed |
| pubmed-article:19771552 | pubmed:author | pubmed-author:ZhangXiulianX | lld:pubmed |
| pubmed-article:19771552 | pubmed:author | pubmed-author:MiyamotoHirom... | lld:pubmed |
| pubmed-article:19771552 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19771552 | pubmed:volume | 9 | lld:pubmed |
| pubmed-article:19771552 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19771552 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19771552 | pubmed:pagination | 5078-89 | lld:pubmed |
| pubmed-article:19771552 | pubmed:meshHeading | pubmed-meshheading:19771552... | lld:pubmed |
| pubmed-article:19771552 | pubmed:meshHeading | pubmed-meshheading:19771552... | lld:pubmed |
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| pubmed-article:19771552 | pubmed:meshHeading | pubmed-meshheading:19771552... | lld:pubmed |
| pubmed-article:19771552 | pubmed:meshHeading | pubmed-meshheading:19771552... | lld:pubmed |
| pubmed-article:19771552 | pubmed:meshHeading | pubmed-meshheading:19771552... | lld:pubmed |
| pubmed-article:19771552 | pubmed:meshHeading | pubmed-meshheading:19771552... | lld:pubmed |
| pubmed-article:19771552 | pubmed:meshHeading | pubmed-meshheading:19771552... | lld:pubmed |
| pubmed-article:19771552 | pubmed:meshHeading | pubmed-meshheading:19771552... | lld:pubmed |
| pubmed-article:19771552 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19771552 | pubmed:articleTitle | Proteomic differential display analysis identified upregulated astrocytic phosphoprotein PEA-15 in human malignant pleural mesothelioma cell lines. | lld:pubmed |
| pubmed-article:19771552 | pubmed:affiliation | Department of Biochemistry and Functional Proteomics, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi, Japan. climates@yamaguchi-u.ac.jp | lld:pubmed |
| pubmed-article:19771552 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19771552 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:8682 | entrezgene:pubmed | pubmed-article:19771552 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:19771552 | lld:entrezgene |
