| pubmed-article:19758310 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19758310 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:19758310 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:19758310 | lifeskim:mentions | umls-concept:C1335858 | lld:lifeskim |
| pubmed-article:19758310 | lifeskim:mentions | umls-concept:C0162597 | lld:lifeskim |
| pubmed-article:19758310 | lifeskim:mentions | umls-concept:C0010031 | lld:lifeskim |
| pubmed-article:19758310 | lifeskim:mentions | umls-concept:C1514559 | lld:lifeskim |
| pubmed-article:19758310 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:19758310 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:19758310 | pubmed:dateCreated | 2009-9-25 | lld:pubmed |
| pubmed-article:19758310 | pubmed:abstractText | Sp1, a transcription factor, is upregulated in keratoconus, a cornea-thinning disease. Keratoconus corneas have also been shown to contain increased levels of degradative enzymes such as cathepsin B and decreased proteinase inhibitors such as alpha1-proteinase inhibitor (alpha1-PI). We transfected cultured human corneal stromal cells to overexpress Sp1. The resulting effects on cathepsin B and alpha1-PI levels as well as the cellular proliferative and apoptotic activities were examined by Western blotting and cytochemical staining. It was found that the Sp1 transfected cells contained a greater amount of cathepsin B than did mock transfected controls. The activity of cathepsin B was also increased. By contrast, the protein level of alpha1-PI was lowered in corneal stromal cells upon Sp1 overexpression. The Sp1-induced alterations thus mimicked closely those observed in keratoconus, supporting the notion that Sp1 upregulation may be a key factor contributing directly to the disease development. Furthermore, the apoptotic activity was unaffected in Sp1 transfectants but the proliferation was inhibited, consistent with the idea that Sp1 may play a role in differentiation of corneal cells. | lld:pubmed |
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| pubmed-article:19758310 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19758310 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19758310 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:19758310 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19758310 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:19758310 | pubmed:issn | 1365-2443 | lld:pubmed |
| pubmed-article:19758310 | pubmed:author | pubmed-author:OnoAA | lld:pubmed |
| pubmed-article:19758310 | pubmed:author | pubmed-author:SugarJoelJ | lld:pubmed |
| pubmed-article:19758310 | pubmed:author | pubmed-author:YueBeatrice... | lld:pubmed |
| pubmed-article:19758310 | pubmed:author | pubmed-author:ShenXiangX | lld:pubmed |
| pubmed-article:19758310 | pubmed:author | pubmed-author:ParkJeong-Seo... | lld:pubmed |
| pubmed-article:19758310 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19758310 | pubmed:volume | 14 | lld:pubmed |
| pubmed-article:19758310 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19758310 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19758310 | pubmed:pagination | 1133-9 | lld:pubmed |
| pubmed-article:19758310 | pubmed:dateRevised | 2010-12-3 | lld:pubmed |
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| pubmed-article:19758310 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19758310 | pubmed:articleTitle | Effects of Sp1 overexpression on cultured human corneal stromal cells. | lld:pubmed |
| pubmed-article:19758310 | pubmed:affiliation | Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago College of Medicine, Chicago, IL, USA. | lld:pubmed |
| pubmed-article:19758310 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19758310 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:19758310 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
| entrez-gene:6667 | entrezgene:pubmed | pubmed-article:19758310 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:19758310 | lld:entrezgene |
