| pubmed-article:19729486 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19729486 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:19729486 | lifeskim:mentions | umls-concept:C0235989 | lld:lifeskim |
| pubmed-article:19729486 | lifeskim:mentions | umls-concept:C1705165 | lld:lifeskim |
| pubmed-article:19729486 | lifeskim:mentions | umls-concept:C1527148 | lld:lifeskim |
| pubmed-article:19729486 | lifeskim:mentions | umls-concept:C1880177 | lld:lifeskim |
| pubmed-article:19729486 | lifeskim:mentions | umls-concept:C2700061 | lld:lifeskim |
| pubmed-article:19729486 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:19729486 | pubmed:dateCreated | 2009-10-7 | lld:pubmed |
| pubmed-article:19729486 | pubmed:abstractText | Diabetic nephropathy is the leading cause of chronic renal failure. Myofibroblasts play a major role in the synthesis and secretion of extracellular matrix in diabetic renal fibrosis. Increasing evidence suggests that endothelial cells may undergo endothelial-myofibroblast transition under physiological and pathophysiological circumstances. Therefore, this study investigates whether endothelial-myofibroblast transition occurs and contributes to the development of diabetic renal interstitial fibrosis. Diabetes was induced by administration of streptozotocin to Tie2-Cre;LoxP-EGFP mice, an endothelial lineage-traceable mouse line generated by crossbreeding B6.Cg-Tg(Tek-cre)12F1v/J mice with B6.Cg-Tg(ACTB-Bgeo/GFP)21Lbe/J mice. The endothelial-myofibroblast transition was also studied in MMECs (a mouse pancreatic microvascular endothelial cell line) and primary cultures of CD31+/EYFP- (enhanced yellow fluorescent protein) endothelial cells isolated from adult normal alpha-smooth muscle actin promoter-driven-EYFP (alpha-SMA/EYFP) mouse kidneys. Confocal microscopy demonstrated that 10.4 +/- 4.2 and 23.5 +/- 7.4% of renal interstitial myofibroblasts (alpha-SMA+) in 1- and 6-month streptozotocin-induced diabetic kidneys were of endothelial origin (EGFP+/alpha-SMA+ cells), compared with just 0.2 +/- 0.1% of myofibroblasts in vehicle-treated Tie2-Cre;LoxP-EGFP mice (P < 0.01). Confocal microscopy and real-time PCR showed that transforming growth factor (TGF)-beta1 induced de novo expression of alpha-SMA and loss of expression of VE-cadherin and CD31 in MMECs and primary cultures of renal endothelial cells in a time- and dose-dependent fashion. These findings demonstrate that the endothelial-myofibroblast transition occurs and contributes to the early development and progression of diabetic renal interstitial fibrosis and suggest that the endothelial-myofibroblast transition may be a therapeutic target. | lld:pubmed |
| pubmed-article:19729486 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19729486 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19729486 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:19729486 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19729486 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:19729486 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19729486 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19729486 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:19729486 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19729486 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19729486 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:19729486 | pubmed:issn | 1525-2191 | lld:pubmed |
| pubmed-article:19729486 | pubmed:author | pubmed-author:BertramJohn... | lld:pubmed |
| pubmed-article:19729486 | pubmed:author | pubmed-author:LiJinhuaJ | lld:pubmed |
| pubmed-article:19729486 | pubmed:author | pubmed-author:YuXindiX | lld:pubmed |
| pubmed-article:19729486 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19729486 | pubmed:volume | 175 | lld:pubmed |
| pubmed-article:19729486 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19729486 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19729486 | pubmed:pagination | 1380-8 | lld:pubmed |
| pubmed-article:19729486 | pubmed:dateRevised | 2010-10-4 | lld:pubmed |
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| pubmed-article:19729486 | pubmed:meshHeading | pubmed-meshheading:19729486... | lld:pubmed |
| pubmed-article:19729486 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19729486 | pubmed:articleTitle | Endothelial-myofibroblast transition contributes to the early development of diabetic renal interstitial fibrosis in streptozotocin-induced diabetic mice. | lld:pubmed |
| pubmed-article:19729486 | pubmed:affiliation | Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria 3800, Australia. jinhua.li@med.monash.edu.au | lld:pubmed |
| pubmed-article:19729486 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19729486 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:19729486 | lld:pubmed |
