| pubmed-article:19727131 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19727131 | lifeskim:mentions | umls-concept:C0017296 | lld:lifeskim |
| pubmed-article:19727131 | lifeskim:mentions | umls-concept:C0078058 | lld:lifeskim |
| pubmed-article:19727131 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:19727131 | lifeskim:mentions | umls-concept:C0598958 | lld:lifeskim |
| pubmed-article:19727131 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:19727131 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:19727131 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:19727131 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:19727131 | pubmed:dateCreated | 2009-11-12 | lld:pubmed |
| pubmed-article:19727131 | pubmed:abstractText | Engineered zinc-finger protein (ZFP) transcription factors induce the expression of endogenous genes and can be remotely delivered using adenoviral vectors. One such factor, Ad-32Ep65-Flag (Ad-p65), targets and induces expression of vascular endothelial growth factor (VEGF; also called VEGF-A) splice variants in their normal biological stoichiometry. We show that Ad-p65 transfection of primary motor neurons results in VEGF variant expression and a significant increase in axon outgrowth in these cells. Given the neuroprotective effects of VEGF and its ability to increase neurite outgrowth, we examined the efficacy of Ad-p65 to enhance motor neuron regeneration in vivo using rats that have undergone recurrent laryngeal nerve (RLN)-crush injury. Injection of Ad-p65 after RLN crush accelerated the return of vocal fold mobility and the percentage of nerve-endplate contacts in the thyroarytenoid muscle. Overall, adenoviral delivery of an engineered ZFP transcription factor inducing VEGF-A splice variant expression enhances nerve regeneration. ZFP transcription factor gene therapy to increase expression of the full complement of VEGF-A splice variants is a promising avenue for the treatment of nerve injury and neurodegeneration. | lld:pubmed |
| pubmed-article:19727131 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19727131 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19727131 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19727131 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19727131 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19727131 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19727131 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19727131 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:19727131 | pubmed:issn | 1476-5462 | lld:pubmed |
| pubmed-article:19727131 | pubmed:author | pubmed-author:FungKK | lld:pubmed |
| pubmed-article:19727131 | pubmed:author | pubmed-author:FeldmanE LEL | lld:pubmed |
| pubmed-article:19727131 | pubmed:author | pubmed-author:HogikyanN DND | lld:pubmed |
| pubmed-article:19727131 | pubmed:author | pubmed-author:SprattS KSK | lld:pubmed |
| pubmed-article:19727131 | pubmed:author | pubmed-author:LUS CSC | lld:pubmed |
| pubmed-article:19727131 | pubmed:author | pubmed-author:LunnJ SJS | lld:pubmed |
| pubmed-article:19727131 | pubmed:author | pubmed-author:SakowskiS ASA | lld:pubmed |
| pubmed-article:19727131 | pubmed:author | pubmed-author:HeavenerS BSB | lld:pubmed |
| pubmed-article:19727131 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19727131 | pubmed:volume | 16 | lld:pubmed |
| pubmed-article:19727131 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19727131 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19727131 | pubmed:pagination | 1292-9 | lld:pubmed |
| pubmed-article:19727131 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:19727131 | pubmed:meshHeading | pubmed-meshheading:19727131... | lld:pubmed |
| pubmed-article:19727131 | pubmed:meshHeading | pubmed-meshheading:19727131... | lld:pubmed |
| pubmed-article:19727131 | pubmed:meshHeading | pubmed-meshheading:19727131... | lld:pubmed |
| pubmed-article:19727131 | pubmed:meshHeading | pubmed-meshheading:19727131... | lld:pubmed |
| pubmed-article:19727131 | pubmed:meshHeading | pubmed-meshheading:19727131... | lld:pubmed |
| pubmed-article:19727131 | pubmed:meshHeading | pubmed-meshheading:19727131... | lld:pubmed |
| pubmed-article:19727131 | pubmed:meshHeading | pubmed-meshheading:19727131... | lld:pubmed |
| pubmed-article:19727131 | pubmed:meshHeading | pubmed-meshheading:19727131... | lld:pubmed |
| pubmed-article:19727131 | pubmed:meshHeading | pubmed-meshheading:19727131... | lld:pubmed |
| pubmed-article:19727131 | pubmed:meshHeading | pubmed-meshheading:19727131... | lld:pubmed |
| pubmed-article:19727131 | pubmed:meshHeading | pubmed-meshheading:19727131... | lld:pubmed |
| pubmed-article:19727131 | pubmed:meshHeading | pubmed-meshheading:19727131... | lld:pubmed |
| pubmed-article:19727131 | pubmed:meshHeading | pubmed-meshheading:19727131... | lld:pubmed |
| pubmed-article:19727131 | pubmed:meshHeading | pubmed-meshheading:19727131... | lld:pubmed |
| pubmed-article:19727131 | pubmed:meshHeading | pubmed-meshheading:19727131... | lld:pubmed |
| pubmed-article:19727131 | pubmed:meshHeading | pubmed-meshheading:19727131... | lld:pubmed |
| pubmed-article:19727131 | pubmed:meshHeading | pubmed-meshheading:19727131... | lld:pubmed |
| pubmed-article:19727131 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19727131 | pubmed:articleTitle | Neuroprotection using gene therapy to induce vascular endothelial growth factor-A expression. | lld:pubmed |
| pubmed-article:19727131 | pubmed:affiliation | Department of Neurology, University of Michigan Medical Center, Ann Arbor, MI, USA. | lld:pubmed |
| pubmed-article:19727131 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19727131 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:19727131 | pubmed:publicationType | Evaluation Studies | lld:pubmed |
| pubmed-article:19727131 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
