| pubmed-article:1967167 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1967167 | lifeskim:mentions | umls-concept:C0580797 | lld:lifeskim |
| pubmed-article:1967167 | lifeskim:mentions | umls-concept:C0032136 | lld:lifeskim |
| pubmed-article:1967167 | lifeskim:mentions | umls-concept:C0017258 | lld:lifeskim |
| pubmed-article:1967167 | lifeskim:mentions | umls-concept:C2700640 | lld:lifeskim |
| pubmed-article:1967167 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:1967167 | pubmed:dateCreated | 1990-2-1 | lld:pubmed |
| pubmed-article:1967167 | pubmed:abstractText | A clone containing the 987P fimbrial gene cluster was selected from a cosmid library of total DNA of the prototype Escherichia coli strain 987 by using 987P-specific antiserum. A subclone of 12 kilobases containing all of the genes required for fimbrial expression on a nonfimbriated K-12 strain of E. coli and a DNA fragment internal to the fimbrial subunit gene were used to probe the prototype strain and various isolates of 987P-fimbriated enterotoxigenic E. coli. All strains had several plasmids, as shown by agarose gel electrophoresis, and each of five strains which expressed 987P fimbriae showed a plasmid of 35 to 40 megadaltons (MDa) hybridizing to both 987P-specific probes. Hybridization to restricted DNA of strain 987 supported a plasmid origin for the cloned 987P gene cluster. Moreover, an isogenic strain which had lost its 35-MDa plasmid was no longer capable of synthesizing fimbrial subunits, but regained fimbrial expression after reintroduction of the TnphoA (Tn5 IS50L::phoA)-tagged 35-MDa plasmid. Absence of fimbrial subunit synthesis in K-12 strains transformed with the 35-MDa plasmid alone suggested the requirement of regulatory elements existing in strain 987 but missing in K-12 strains. A probe for the heat-stable enterotoxin STIa hybridized in each of the 987P-fimbriated strains to the plasmid containing the 987P genes and in most of these strains to an additional plasmid which contained the gene for the heat-stable enterotoxin STII. Occurrence of the 987P and STIa genes on the same replicon correlates with epidemiological observations, STIa being the most prevalent toxin produced by 987P-fimbriated E. coli. | lld:pubmed |
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| pubmed-article:1967167 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:1967167 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:1967167 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1967167 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:1967167 | pubmed:issn | 0019-9567 | lld:pubmed |
| pubmed-article:1967167 | pubmed:author | pubmed-author:BeacheyE HEH | lld:pubmed |
| pubmed-article:1967167 | pubmed:author | pubmed-author:TaylorR KRK | lld:pubmed |
| pubmed-article:1967167 | pubmed:author | pubmed-author:SchifferliD... | lld:pubmed |
| pubmed-article:1967167 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1967167 | pubmed:volume | 58 | lld:pubmed |
| pubmed-article:1967167 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1967167 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1967167 | pubmed:pagination | 149-56 | lld:pubmed |
| pubmed-article:1967167 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:1967167 | pubmed:meshHeading | pubmed-meshheading:1967167-... | lld:pubmed |
| pubmed-article:1967167 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:1967167 | pubmed:articleTitle | The 987P fimbrial gene cluster of enterotoxigenic Escherichia coli is plasmid encoded. | lld:pubmed |
| pubmed-article:1967167 | pubmed:affiliation | Department of Microbiology and Immunology, University of Tennessee Center for the Health Sciences, Memphis. | lld:pubmed |
| pubmed-article:1967167 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1967167 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1967167 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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