| pubmed-article:1967051 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1967051 | lifeskim:mentions | umls-concept:C1138842 | lld:lifeskim |
| pubmed-article:1967051 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:1967051 | lifeskim:mentions | umls-concept:C0035687 | lld:lifeskim |
| pubmed-article:1967051 | lifeskim:mentions | umls-concept:C0002085 | lld:lifeskim |
| pubmed-article:1967051 | lifeskim:mentions | umls-concept:C0040649 | lld:lifeskim |
| pubmed-article:1967051 | lifeskim:mentions | umls-concept:C0524865 | lld:lifeskim |
| pubmed-article:1967051 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:1967051 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:1967051 | pubmed:dateCreated | 1992-10-29 | lld:pubmed |
| pubmed-article:1967051 | pubmed:abstractText | The maize Bronze-2 (Bz2) gene, whose product acts late in the anthocyanin biosynthetic pathway, has been cloned and its transcript has been mapped. We have developed a general procedure for reconstructing wild-type alleles from transposable element-induced mutants. An existing transposon-containing clone, bz2::mu1 [McLaughlin, M., and Walbot, V. (1987). Genetics 117, 771-776], was modified by replacing the region of bz2::mu1 containing the transposon with the corresponding polymerase chain reaction-amplified sequence from the progenitor allele that has no Mu insertion. Particle gun delivery of the reconstructed Bz2 gene to embryonic scutellar tissue lacking a functional Bz2 gene complemented the bz2 mutant phenotype, as demonstrated by the production of purple spots. Having cloned the wild-type allele, we then analyzed the Bz2 transcript, whose features include an 82-nucleotide 5'-untranslated leader, one small intron (78 base pairs) within the coding region, and multiple polyadenylation sites. Four Mutator transposon insertions that eliminate gene function were mapped within the 850-nucleotide transcription unit. We found that variable levels of unspliced Bz2 RNA are present in purple husk tissue; this finding may indicate that the expression of Bz2 is regulated in part at the level of transcript processing. | lld:pubmed |
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| pubmed-article:1967051 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1967051 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1967051 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:1967051 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1967051 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:1967051 | pubmed:issn | 1040-4651 | lld:pubmed |
| pubmed-article:1967051 | pubmed:author | pubmed-author:WalbotVV | lld:pubmed |
| pubmed-article:1967051 | pubmed:author | pubmed-author:NashJJ | lld:pubmed |
| pubmed-article:1967051 | pubmed:author | pubmed-author:LuehrsenK RKR | lld:pubmed |
| pubmed-article:1967051 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1967051 | pubmed:volume | 2 | lld:pubmed |
| pubmed-article:1967051 | pubmed:geneSymbol | Bz2 | lld:pubmed |
| pubmed-article:1967051 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1967051 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1967051 | pubmed:pagination | 1039-49 | lld:pubmed |
| pubmed-article:1967051 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:1967051 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:1967051 | pubmed:articleTitle | Bronze-2 gene of maize: reconstruction of a wild-type allele and analysis of transcription and splicing. | lld:pubmed |
| pubmed-article:1967051 | pubmed:affiliation | Department of Biological Sciences, Stanford University, California 94305-5020. | lld:pubmed |
| pubmed-article:1967051 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1967051 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1967051 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:1967051 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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