pubmed-article:19666812 | pubmed:abstractText | A Gram-positive actinobacterium (CC-NMPT-T3(T)) was isolated from iron-ore-contaminated soil near New Mangalore Port, Karnataka, India. Based on 16S rRNA gene sequence similarity studies, strain CC-NMPT-T3(T) belongs to the genus Georgenia and is most closely related to Georgenia muralis (98.6 %), Georgenia ruanii (97.4 %) and Georgenia thermotolerans (97.4 %). The peptidoglycan is of the type A4alpha l-Lys<--l-Glu. The predominant isoprenoid quinone is menaquinone MK-8(H(4)) and the polar lipid profile is composed of the predominant compound diphosphatidylglycerol, moderate amounts of a phosphatidylinositol-mannoside, phosphatidylinositol and minor amounts of another phosphatidylmannoside and phosphatidylglycerol. The major fatty acids of strain CC-NMPT-T3(T) are anteiso-C(15 : 0) and iso-C(15 : 0). The results of DNA-DNA hybridizations, and physiological and biochemical tests allowed a clear phenotypic differentiation of strain CC-NMPT-T3(T) from all other Georgenia species. Strain CC-NMPT-T3(T) represents a novel species, for which the name Georgenia soli sp. nov. is proposed. The type strain is CC-NMPT-T3(T) (=DSM 21838(T)=CCM 7658(T)). | lld:pubmed |