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pubmed-article:19665398pubmed:dateCreated2010-10-25lld:pubmed
pubmed-article:19665398pubmed:abstractTextOf 146 dogs from a visceral leishmaniosis-endemic area that tested seronegative by indirect immunofluorescence (IIF) on blood samples collected on filter paper (IIFp), 51 (34.9%) and 10 (6.8%) tested positive by IIF on serum samples (IIFs) and enzyme immunoassay, respectively. Three samples (2.0%) tested positive by PCR. Leishmania chagasi was isolated from the skin of five (3.4%) dogs. Amastigote forms were identified in two of these five animals following histopathological and immunohistochemical examination. The findings highlight that detection methods such as IIFp can permit dogs infected with L. chagasi to remain undetected in endemic areas with attendant consequences for the epidemiology of infection both in the canine and human populations.lld:pubmed
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pubmed-article:19665398pubmed:authorpubmed-author:PachecoR SRSlld:pubmed
pubmed-article:19665398pubmed:authorpubmed-author:PiresM QMQlld:pubmed
pubmed-article:19665398pubmed:authorpubmed-author:PintoA GAGlld:pubmed
pubmed-article:19665398pubmed:authorpubmed-author:MenezesR CRClld:pubmed
pubmed-article:19665398pubmed:authorpubmed-author:FurtadoM CMClld:pubmed
pubmed-article:19665398pubmed:authorpubmed-author:MadeiraM FMFlld:pubmed
pubmed-article:19665398pubmed:authorpubmed-author:SchubachT M...lld:pubmed
pubmed-article:19665398pubmed:authorpubmed-author:FigueiredoF...lld:pubmed
pubmed-article:19665398pubmed:copyrightInfoCopyright © 2009 Elsevier Ltd. All rights reserved.lld:pubmed
pubmed-article:19665398pubmed:issnTypeElectroniclld:pubmed
pubmed-article:19665398pubmed:volume186lld:pubmed
pubmed-article:19665398pubmed:ownerNLMlld:pubmed
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pubmed-article:19665398pubmed:year2010lld:pubmed
pubmed-article:19665398pubmed:articleTitleEfficacy of an indirect immunofluorescence test in the diagnosis of canine leishmaniosis.lld:pubmed
pubmed-article:19665398pubmed:affiliationLaboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brazil. fabiano.figueiredo@ipec.fiocruz.brlld:pubmed
pubmed-article:19665398pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:19665398pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
pubmed-article:19665398pubmed:publicationTypeEvaluation Studieslld:pubmed