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pubmed-article:19654045pubmed:abstractTextVoxelation creates expression atlases by high-throughput analysis of spatially registered cubes or voxels harvested from the brain. The modality independence of voxelation allows a variety of bioanalytical techniques to be used to map abundance. Protein expression patterns in the brain can be obtained using liquid chromatography (LC) combined with mass spectrometry (MS). Here we describe the methodology of voxelation as it pertains particularly to LC-MS proteomic analysis: sample preparation, instrumental set up and analysis, peptide identification and protein relative abundance quantitation. We also briefly describe some of the advantages, limitations and insights into the brain that can be obtained using combined proteomic and transcriptomic maps.lld:pubmed
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pubmed-article:19654045pubmed:copyrightInfoCopyright 2009 Elsevier Inc. All rights reserved.lld:pubmed
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pubmed-article:19654045pubmed:articleTitleMapping protein abundance patterns in the brain using voxelation combined with liquid chromatography and mass spectrometry.lld:pubmed
pubmed-article:19654045pubmed:affiliationBiological Sciences Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA 99352, USA.lld:pubmed
pubmed-article:19654045pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:19654045pubmed:publicationTypeResearch Support, U.S. Gov't, Non-P.H.S.lld:pubmed
pubmed-article:19654045pubmed:publicationTypeResearch Support, N.I.H., Extramurallld:pubmed
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