| pubmed-article:19651878 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19651878 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:19651878 | lifeskim:mentions | umls-concept:C0040711 | lld:lifeskim |
| pubmed-article:19651878 | lifeskim:mentions | umls-concept:C0074289 | lld:lifeskim |
| pubmed-article:19651878 | lifeskim:mentions | umls-concept:C1521761 | lld:lifeskim |
| pubmed-article:19651878 | lifeskim:mentions | umls-concept:C0678594 | lld:lifeskim |
| pubmed-article:19651878 | lifeskim:mentions | umls-concept:C0013879 | lld:lifeskim |
| pubmed-article:19651878 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:19651878 | lifeskim:mentions | umls-concept:C0041573 | lld:lifeskim |
| pubmed-article:19651878 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:19651878 | pubmed:issue | 17 | lld:pubmed |
| pubmed-article:19651878 | pubmed:dateCreated | 2009-10-14 | lld:pubmed |
| pubmed-article:19651878 | pubmed:abstractText | The selenocysteine insertion sequence (SECIS) element directs the translational recoding of UGA as selenocysteine. In eukaryotes, the SECIS is located downstream of the UGA codon in the 3'-UTR of the selenoprotein mRNA. Despite poor sequence conservation, all SECIS elements form a similar stem-loop structure containing a putative kink-turn motif. We functionally characterized the 26 SECIS elements encoded in the human genome. Surprisingly, the SECIS elements displayed a wide range of UGA recoding activities, spanning several 1000-fold in vivo and several 100-fold in vitro. The difference in activity between a representative strong and weak SECIS element was not explained by differential binding affinity of SECIS binding Protein 2, a limiting factor for selenocysteine incorporation. Using chimeric SECIS molecules, we identified the internal loop and helix 2, which flank the kink-turn motif, as critical determinants of UGA recoding activity. The simultaneous presence of a GC base pair in helix 2 and a U in the 5'-side of the internal loop was a statistically significant predictor of weak recoding activity. Thus, the SECIS contains intrinsic information that modulates selenocysteine incorporation efficiency. | lld:pubmed |
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| pubmed-article:19651878 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19651878 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19651878 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19651878 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:19651878 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19651878 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:19651878 | pubmed:issn | 1362-4962 | lld:pubmed |
| pubmed-article:19651878 | pubmed:author | pubmed-author:Jean-JeanOliv... | lld:pubmed |
| pubmed-article:19651878 | pubmed:author | pubmed-author:DriscollDonna... | lld:pubmed |
| pubmed-article:19651878 | pubmed:author | pubmed-author:ChavatteLaure... | lld:pubmed |
| pubmed-article:19651878 | pubmed:author | pubmed-author:LatrècheLynda... | lld:pubmed |
| pubmed-article:19651878 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19651878 | pubmed:volume | 37 | lld:pubmed |
| pubmed-article:19651878 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19651878 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19651878 | pubmed:pagination | 5868-80 | lld:pubmed |
| pubmed-article:19651878 | pubmed:dateRevised | 2010-9-27 | lld:pubmed |
| pubmed-article:19651878 | pubmed:meshHeading | pubmed-meshheading:19651878... | lld:pubmed |
| pubmed-article:19651878 | pubmed:meshHeading | pubmed-meshheading:19651878... | lld:pubmed |
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| pubmed-article:19651878 | pubmed:meshHeading | pubmed-meshheading:19651878... | lld:pubmed |
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| pubmed-article:19651878 | pubmed:meshHeading | pubmed-meshheading:19651878... | lld:pubmed |
| pubmed-article:19651878 | pubmed:meshHeading | pubmed-meshheading:19651878... | lld:pubmed |
| pubmed-article:19651878 | pubmed:meshHeading | pubmed-meshheading:19651878... | lld:pubmed |
| pubmed-article:19651878 | pubmed:meshHeading | pubmed-meshheading:19651878... | lld:pubmed |
| pubmed-article:19651878 | pubmed:meshHeading | pubmed-meshheading:19651878... | lld:pubmed |
| pubmed-article:19651878 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19651878 | pubmed:articleTitle | Novel structural determinants in human SECIS elements modulate the translational recoding of UGA as selenocysteine. | lld:pubmed |
