Linked Life Data

19638344

Source:http://linkedlifedata.com/resource/pubmed/id/19638344

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:19638344rdf:typepubmed:Citationlld:pubmed
pubmed-article:19638344lifeskim:mentionsumls-concept:C0086418lld:lifeskim
pubmed-article:19638344lifeskim:mentionsumls-concept:C0020792lld:lifeskim
pubmed-article:19638344lifeskim:mentionsumls-concept:C0034656lld:lifeskim
pubmed-article:19638344lifeskim:mentionsumls-concept:C0024623lld:lifeskim
pubmed-article:19638344lifeskim:mentionsumls-concept:C0334227lld:lifeskim
pubmed-article:19638344lifeskim:mentionsumls-concept:C0017337lld:lifeskim
pubmed-article:19638344lifeskim:mentionsumls-concept:C0014582lld:lifeskim
pubmed-article:19638344lifeskim:mentionsumls-concept:C1099354lld:lifeskim
pubmed-article:19638344lifeskim:mentionsumls-concept:C0599776lld:lifeskim
pubmed-article:19638344lifeskim:mentionsumls-concept:C1414990lld:lifeskim
pubmed-article:19638344pubmed:issue39lld:pubmed
pubmed-article:19638344pubmed:dateCreated2009-9-21lld:pubmed
pubmed-article:19638344pubmed:abstractTextEpirubicin has been widely used for chemotherapeutic treatment of gastric cancer; however, intrinsic and acquired chemoresistance remains an obstacle to successful management. The mechanisms underlying epirubicin resistance are still not well defined. Here we report the construction and application of a partially randomized retrovirus library of 4 x 10(6) small interfering RNAs to identify novel genes whose suppression confers epirubicin resistance in gastric cancer cells SGC7901. From 12 resistant cell colonies, two small interfering RNAs targeting GAS1 (growth arrest-specific 1) and PTEN (phosphatase and tensin homolog), respectively, were identified and validated. We identified a previously unrecognized chemoresistance role for GAS1. GAS1 suppression resulted in significant epirubicin resistance and cross-resistance to 5-fluorouracil and cisplatin in various gastric cancer cell lines. GAS1 suppression promoted multidrug resistance through apoptosis inhibition, partially by up-regulating the Bcl-2/Bax ratio that was abolished by Bcl-2 inhibition. GAS1 suppression induced chemoresistance partially by increasing drug efflux in an ATP-binding cassette transporter and drug-dependent manner. P-glycoprotein (P-gp) and BCRP (breast cancer resistance protein) but not MRP-1 were up-regulated, and targeted knockdown of P-gp and BCRP could partially reverse GAS1 suppression-induced epirubicin resistance. Verapamil, a P-gp inhibitor, could reverse P-gp substrate (epirubicin) but not non-P-gp substrate (5-fluorouracil and cisplatin) resistance in GAS1-suppressed gastric cancer cells. BCRP down-regulation could partially reverse 5-fluorouracil but not cisplatin resistance induced by GAS1 suppression, suggesting 5-fluorouracil but not cisplatin was a BCRP substrate. These results suggest that GAS1 might be a target to overcome multidrug resistance and provide a novel approach to identifying candidate genes that suppress chemoresistance of gastric cancers.lld:pubmed
pubmed-article:19638344pubmed:languageenglld:pubmed
pubmed-article:19638344pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19638344pubmed:citationSubsetIMlld:pubmed
pubmed-article:19638344pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19638344pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19638344pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19638344pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19638344pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19638344pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19638344pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19638344pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19638344pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19638344pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19638344pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19638344pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19638344pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19638344pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19638344pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19638344pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19638344pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19638344pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19638344pubmed:statusMEDLINElld:pubmed
pubmed-article:19638344pubmed:monthSeplld:pubmed
pubmed-article:19638344pubmed:issn1083-351Xlld:pubmed
pubmed-article:19638344pubmed:authorpubmed-author:KimW EWElld:pubmed
pubmed-article:19638344pubmed:authorpubmed-author:FanDaimingDlld:pubmed
pubmed-article:19638344pubmed:authorpubmed-author:LinXiaXlld:pubmed
pubmed-article:19638344pubmed:authorpubmed-author:GangYiYlld:pubmed
pubmed-article:19638344pubmed:authorpubmed-author:LiuZhiguoZlld:pubmed
pubmed-article:19638344pubmed:authorpubmed-author:HonghongWangWlld:pubmed
pubmed-article:19638344pubmed:authorpubmed-author:YaoLipingLlld:pubmed
pubmed-article:19638344pubmed:authorpubmed-author:ZhangYongguoYlld:pubmed
pubmed-article:19638344pubmed:authorpubmed-author:QiaoTaidongTlld:pubmed
pubmed-article:19638344pubmed:authorpubmed-author:LiTingtingTlld:pubmed
pubmed-article:19638344pubmed:authorpubmed-author:PanYanglinYlld:pubmed
pubmed-article:19638344pubmed:authorpubmed-author:JinHaifengHlld:pubmed
pubmed-article:19638344pubmed:authorpubmed-author:ZhaoLinaLlld:pubmed
pubmed-article:19638344pubmed:authorpubmed-author:HeLijieLlld:pubmed
pubmed-article:19638344pubmed:issnTypeElectroniclld:pubmed
pubmed-article:19638344pubmed:day25lld:pubmed
pubmed-article:19638344pubmed:volume284lld:pubmed
pubmed-article:19638344pubmed:ownerNLMlld:pubmed
pubmed-article:19638344pubmed:authorsCompleteYlld:pubmed
pubmed-article:19638344pubmed:pagination26273-85lld:pubmed
pubmed-article:19638344pubmed:dateRevised2010-11-18lld:pubmed
pubmed-article:19638344pubmed:meshHeadingpubmed-meshheading:19638344...lld:pubmed
pubmed-article:19638344pubmed:meshHeadingpubmed-meshheading:19638344...lld:pubmed
pubmed-article:19638344pubmed:meshHeadingpubmed-meshheading:19638344...lld:pubmed
pubmed-article:19638344pubmed:meshHeadingpubmed-meshheading:19638344...lld:pubmed
pubmed-article:19638344pubmed:meshHeadingpubmed-meshheading:19638344...lld:pubmed
pubmed-article:19638344pubmed:meshHeadingpubmed-meshheading:19638344...lld:pubmed
pubmed-article:19638344pubmed:meshHeadingpubmed-meshheading:19638344...lld:pubmed
pubmed-article:19638344pubmed:meshHeadingpubmed-meshheading:19638344...lld:pubmed
pubmed-article:19638344pubmed:meshHeadingpubmed-meshheading:19638344...lld:pubmed
pubmed-article:19638344pubmed:meshHeadingpubmed-meshheading:19638344...lld:pubmed
pubmed-article:19638344pubmed:meshHeadingpubmed-meshheading:19638344...lld:pubmed
pubmed-article:19638344pubmed:meshHeadingpubmed-meshheading:19638344...lld:pubmed
pubmed-article:19638344pubmed:meshHeadingpubmed-meshheading:19638344...lld:pubmed
pubmed-article:19638344pubmed:meshHeadingpubmed-meshheading:19638344...lld:pubmed
pubmed-article:19638344pubmed:meshHeadingpubmed-meshheading:19638344...lld:pubmed
pubmed-article:19638344pubmed:meshHeadingpubmed-meshheading:19638344...lld:pubmed
pubmed-article:19638344pubmed:meshHeadingpubmed-meshheading:19638344...lld:pubmed
pubmed-article:19638344pubmed:meshHeadingpubmed-meshheading:19638344...lld:pubmed
pubmed-article:19638344pubmed:meshHeadingpubmed-meshheading:19638344...lld:pubmed
pubmed-article:19638344pubmed:meshHeadingpubmed-meshheading:19638344...lld:pubmed
pubmed-article:19638344pubmed:meshHeadingpubmed-meshheading:19638344...lld:pubmed
pubmed-article:19638344pubmed:meshHeadingpubmed-meshheading:19638344...lld:pubmed
pubmed-article:19638344pubmed:meshHeadingpubmed-meshheading:19638344...lld:pubmed
pubmed-article:19638344pubmed:meshHeadingpubmed-meshheading:19638344...lld:pubmed
pubmed-article:19638344pubmed:meshHeadingpubmed-meshheading:19638344...lld:pubmed
pubmed-article:19638344pubmed:meshHeadingpubmed-meshheading:19638344...lld:pubmed
pubmed-article:19638344pubmed:meshHeadingpubmed-meshheading:19638344...lld:pubmed
pubmed-article:19638344pubmed:meshHeadingpubmed-meshheading:19638344...lld:pubmed
pubmed-article:19638344pubmed:year2009lld:pubmed
pubmed-article:19638344pubmed:articleTitleIdentification of GAS1 as an epirubicin resistance-related gene in human gastric cancer cells with a partially randomized small interfering RNA library.lld:pubmed
pubmed-article:19638344pubmed:affiliationState Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an 710032, China.lld:pubmed
pubmed-article:19638344pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:19638344pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
entrez-gene:2619entrezgene:pubmedpubmed-article:19638344lld:entrezgene
http://linkedlifedata.com/r...entrezgene:pubmedpubmed-article:19638344lld:entrezgene
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:19638344lld:pubmed