| pubmed-article:19638322 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19638322 | lifeskim:mentions | umls-concept:C0023290 | lld:lifeskim |
| pubmed-article:19638322 | lifeskim:mentions | umls-concept:C0003320 | lld:lifeskim |
| pubmed-article:19638322 | lifeskim:mentions | umls-concept:C0178539 | lld:lifeskim |
| pubmed-article:19638322 | lifeskim:mentions | umls-concept:C1704632 | lld:lifeskim |
| pubmed-article:19638322 | lifeskim:mentions | umls-concept:C0871261 | lld:lifeskim |
| pubmed-article:19638322 | lifeskim:mentions | umls-concept:C0023273 | lld:lifeskim |
| pubmed-article:19638322 | lifeskim:mentions | umls-concept:C2911692 | lld:lifeskim |
| pubmed-article:19638322 | lifeskim:mentions | umls-concept:C1706817 | lld:lifeskim |
| pubmed-article:19638322 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:19638322 | lifeskim:mentions | umls-concept:C1517004 | lld:lifeskim |
| pubmed-article:19638322 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:19638322 | pubmed:dateCreated | 2009-10-13 | lld:pubmed |
| pubmed-article:19638322 | pubmed:abstractText | The potential of autoclaved and heat-killed antigen of Leishmania donovani to induce cell-mediated and humoral response has been evaluated in the present study. The vaccines were delivered thrice subcutaneously at an interval of 2 weeks. Two weeks after second booster, BALB/c mice were challenged with 10(7) stationary phase promastigotes of L. donovani. Significant protection was achieved in immunized mice against L. donovani challenge with 69% to 76% and 59% to 64% reduction in parasite load in the liver and spleen respectively. Immunization induced significantly higher level of delayed type hypersensitivity (DTH) response in mice immunized with heat-killed antigen followed by autoclaved antigen. The immune response was assessed by quantifying Leishmania-specific antibodies and cytokine production. The antibody response was predominantly of IgG type with increased IgG2a production and lesser amount of IgM. The immunization preferentially stimulates the production of IFN-gamma and IL-2 in splenocytes which suggests a Th1 type response with a concomitant down-regulation of IL-10 and IL-4. These results indicate a potential for the heat-killed and autoclaved antigen as a vaccine which could trigger cell-mediated immune response. | lld:pubmed |
| pubmed-article:19638322 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19638322 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19638322 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19638322 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19638322 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19638322 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19638322 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19638322 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19638322 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19638322 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:19638322 | pubmed:issn | 1873-0329 | lld:pubmed |
| pubmed-article:19638322 | pubmed:author | pubmed-author:SharmaMeenaks... | lld:pubmed |
| pubmed-article:19638322 | pubmed:author | pubmed-author:KaurSukhbirS | lld:pubmed |
| pubmed-article:19638322 | pubmed:author | pubmed-author:NagillRajeevR | lld:pubmed |
| pubmed-article:19638322 | pubmed:author | pubmed-author:MahajanRamanR | lld:pubmed |
| pubmed-article:19638322 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19638322 | pubmed:volume | 58 | lld:pubmed |
| pubmed-article:19638322 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19638322 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19638322 | pubmed:pagination | 359-66 | lld:pubmed |
| pubmed-article:19638322 | pubmed:meshHeading | pubmed-meshheading:19638322... | lld:pubmed |
| pubmed-article:19638322 | pubmed:meshHeading | pubmed-meshheading:19638322... | lld:pubmed |
| pubmed-article:19638322 | pubmed:meshHeading | pubmed-meshheading:19638322... | lld:pubmed |
| pubmed-article:19638322 | pubmed:meshHeading | pubmed-meshheading:19638322... | lld:pubmed |
| pubmed-article:19638322 | pubmed:meshHeading | pubmed-meshheading:19638322... | lld:pubmed |
| pubmed-article:19638322 | pubmed:meshHeading | pubmed-meshheading:19638322... | lld:pubmed |
| pubmed-article:19638322 | pubmed:meshHeading | pubmed-meshheading:19638322... | lld:pubmed |
| pubmed-article:19638322 | pubmed:meshHeading | pubmed-meshheading:19638322... | lld:pubmed |
| pubmed-article:19638322 | pubmed:meshHeading | pubmed-meshheading:19638322... | lld:pubmed |
| pubmed-article:19638322 | pubmed:meshHeading | pubmed-meshheading:19638322... | lld:pubmed |
| pubmed-article:19638322 | pubmed:meshHeading | pubmed-meshheading:19638322... | lld:pubmed |
| pubmed-article:19638322 | pubmed:meshHeading | pubmed-meshheading:19638322... | lld:pubmed |
| pubmed-article:19638322 | pubmed:meshHeading | pubmed-meshheading:19638322... | lld:pubmed |
| pubmed-article:19638322 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19638322 | pubmed:articleTitle | Induction of cellular and humoral responses by autoclaved and heat-killed antigen of Leishmania donovani in experimental visceral leishmaniasis. | lld:pubmed |
| pubmed-article:19638322 | pubmed:affiliation | Parasitology Laboratory, Department of Zoology, Panjab University, Chandigarh-160014, India. | lld:pubmed |
| pubmed-article:19638322 | pubmed:publicationType | Journal Article | lld:pubmed |
