pubmed-article:19628694 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:19628694 | lifeskim:mentions | umls-concept:C0042760 | lld:lifeskim |
pubmed-article:19628694 | lifeskim:mentions | umls-concept:C0019704 | lld:lifeskim |
pubmed-article:19628694 | lifeskim:mentions | umls-concept:C0019425 | lld:lifeskim |
pubmed-article:19628694 | lifeskim:mentions | umls-concept:C0035668 | lld:lifeskim |
pubmed-article:19628694 | lifeskim:mentions | umls-concept:C0017428 | lld:lifeskim |
pubmed-article:19628694 | lifeskim:mentions | umls-concept:C0013682 | lld:lifeskim |
pubmed-article:19628694 | lifeskim:mentions | umls-concept:C0030176 | lld:lifeskim |
pubmed-article:19628694 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
pubmed-article:19628694 | lifeskim:mentions | umls-concept:C0205171 | lld:lifeskim |
pubmed-article:19628694 | lifeskim:mentions | umls-concept:C1522492 | lld:lifeskim |
pubmed-article:19628694 | pubmed:issue | 32 | lld:pubmed |
pubmed-article:19628694 | pubmed:dateCreated | 2009-8-26 | lld:pubmed |
pubmed-article:19628694 | pubmed:abstractText | A long-standing question in retrovirus biology is how RNA genomes are distributed among virions. In the studies presented in this report, we addressed this issue by directly examining HIV-1 RNAs in virions using a modified HIV-1 genome that contained recognition sites for BglG, an antitermination protein in the Escherichia coli bgl operon, which was coexpressed with a fragment of BglG RNA binding protein fused to a fluorescent protein. Our results demonstrate that the majority of virions (>90%) contain viral RNAs. We also coexpressed HIV-1 genomes containing binding sites for BglG or the bacteriophage MS2 coat protein along with 2 fluorescent protein-tagged RNA binding proteins. This method allows simultaneously labeling and discrimination of 2 different RNAs at single-RNA-detection sensitivity. Using this strategy, we obtained physical evidence that virions contain RNAs derived from different parental viruses (heterozygous virion) at ratios expected from a random distribution, and we found that this ratio can be altered by changing the dimerization sequences. Our studies of heterozygous virions also support a generally accepted but unproven assumption that most particles contain 1 dimer. This study provides answers to long-standing questions in HIV-1 biology and illustrates the power and sensitivity of the 2-RNA labeling method, which can also be adapted to analyze various issues of RNA biogenesis including the detection of different RNAs in live cell imaging. | lld:pubmed |
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pubmed-article:19628694 | pubmed:language | eng | lld:pubmed |
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pubmed-article:19628694 | pubmed:citationSubset | IM | lld:pubmed |
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pubmed-article:19628694 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:19628694 | pubmed:month | Aug | lld:pubmed |
pubmed-article:19628694 | pubmed:issn | 1091-6490 | lld:pubmed |
pubmed-article:19628694 | pubmed:author | pubmed-author:LDD | lld:pubmed |
pubmed-article:19628694 | pubmed:author | pubmed-author:SinghJatinder... | lld:pubmed |
pubmed-article:19628694 | pubmed:author | pubmed-author:WrightAndrewA | lld:pubmed |
pubmed-article:19628694 | pubmed:author | pubmed-author:ChenJianboJ | lld:pubmed |
pubmed-article:19628694 | pubmed:author | pubmed-author:HuWei-ShauWS | lld:pubmed |
pubmed-article:19628694 | pubmed:author | pubmed-author:PathakVinay... | lld:pubmed |
pubmed-article:19628694 | pubmed:author | pubmed-author:LockettStephe... | lld:pubmed |
pubmed-article:19628694 | pubmed:author | pubmed-author:CoffinJohn... | lld:pubmed |
pubmed-article:19628694 | pubmed:author | pubmed-author:NikolaitchikO... | lld:pubmed |
pubmed-article:19628694 | pubmed:author | pubmed-author:BencsicsCraig... | lld:pubmed |
pubmed-article:19628694 | pubmed:issnType | Electronic | lld:pubmed |
pubmed-article:19628694 | pubmed:day | 11 | lld:pubmed |
pubmed-article:19628694 | pubmed:volume | 106 | lld:pubmed |
pubmed-article:19628694 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:19628694 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:19628694 | pubmed:pagination | 13535-40 | lld:pubmed |
pubmed-article:19628694 | pubmed:dateRevised | 2010-8-12 | lld:pubmed |
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pubmed-article:19628694 | pubmed:year | 2009 | lld:pubmed |
pubmed-article:19628694 | pubmed:articleTitle | High efficiency of HIV-1 genomic RNA packaging and heterozygote formation revealed by single virion analysis. | lld:pubmed |
pubmed-article:19628694 | pubmed:affiliation | HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702, USA. | lld:pubmed |
pubmed-article:19628694 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:19628694 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
pubmed-article:19628694 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
pubmed-article:19628694 | pubmed:publicationType | Research Support, N.I.H., Intramural | lld:pubmed |
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